Is Physical Activity Associated With the Tethering and Migration of Pro-inflammatory Monocytes in White European and South Asian Males With and Without Central Obesity.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Number of classical monocytes that migrated.
研究概览
简要总结
Being south Asian or centrally obese may be associated with an increased risk of inflammation. The investigators are seeking to investigate whether this is the case by recruiting white European and south Asian men who are lean or have central obesity. Further, the investigators wish to investigate whether physical activity influences the associations.
详细描述
Central obesity is associated with an increased risk of cardiovascular disease. Further, south Asians have been shown to be at an increased risk of cardiovascular disease compared to white Europeans.
Cardiovascular disease is underpinned by inflammation. Evidence suggests that people with obesity have a more pro-inflammatory and pro-migratory monocyte profile compared with individuals who are lean. The excessive monocyte migration contributes to metabolic dysfunction over time, increasing the risk of chronic disease. However, there is no evidence in south Asians.
One modifiable risk factor which may be able to influence this is physical inactivity, with higher levels of physical activity being associated with reduced inflammation. However, although south Asians are more at risk of cardiovascular disease than white Europeans, evidence suggests south Asians are also less physically active than white Europeans.
The investigators are looking to recruit south Asian and white European men who are lean or have central obesity to investigate 1) is there an association between ethnicity and the tethering and migration of pro-inflammatory monocytes? 2) is there an association between central obesity and the tethering and migration of pro-inflammatory monocytes, and is there an interaction with ethnicity? 3) do higher levels of physical activity influence the tethering and migration of pro-inflammatory monocytes, and is this influenced by ethnicity or central obesity?
To investigate this, the investigators are looking to recruit south Asian and white European men who are either centrally obese or lean. The investigators require 1 blood sample and the participants to wear an activity monitor for 7 days.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Non-smokers (including vaping)
- •Not currently dieting
排除标准
- •Musculoskeletal injury that has affected normal ambulation within the last month;
- •Any muscle or bone injuries that influence physical activity
- •Free from heart conditions and blood disorders
- •Weight fluctuation greater than 3kg in the previous 3 months
- •Taking anti-inflammatory medication
结局指标
主要结局
Number of classical monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of non-classical monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Concentrations of classical monocytes.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as cells/uL.
Concentrations of intermediate monocytes.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as cells/uL.
Concentrations of non-classical monocytes.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as cells/uL.
Concentrations of CCR2+ monocytes.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as cells/uL.
Concentrations of CCR2+ classical monocytes.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as cells/uL.
Concentrations of CCR5+ monocytes.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as cells/uL.
Number of intermediate monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of intermediate monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of non-classical monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of CCR5+ monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
CCR2+ receptor expression.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry.
Number of monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of CCR2+ classical monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of classical monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of CCR2+ classical monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of CCR2+ monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of CCR5+ monocytes that migrated.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
Number of CCR2+ monocytes that tethered.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry. Presented as number of cells.
CCR5+ receptor expression.
时间窗: The outcome will be measured as a single-time point assessment in a fasted state on day 1.
Determined via flow cytometry.
次要结局
- Concentration of triacylglycerol.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of c-reactive protein.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of high-density lipoprotein cholesterol (HDL).(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Waist circumference(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Systolic blood pressure.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Diastolic blood pressure.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of total cholesterol.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of low-density lipoprotein cholesterol (LDL).(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Body fat percentage.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of glucose.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of interleukin-6.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Concentration of non-esterified free fatty acids.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Lean mass.(The outcome will be measured as a single-time point assessment in a fasted state on day 1.)
- Light physical activity minutes per day.(Over 7 days +/- the assessment day)
- Moderate-to-vigorous activity minutes per day.(Over 7 days +/- the assessment day)
- Daily steps.(Over 7 days +/- the assessment day)
研究者
Nicolette Bishop
Professor
Loughborough University