Isolation and Characterization of ERBB Expressing Human Heart Progenitor Cells
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- The number of highly proliferative clones isolated
研究概览
简要总结
This study examines the role of the epidermal growth factor (EGF) receptor family and the EGF family of ligands in the regulation of non-myocytes isolated from the human heart.
详细描述
The EGF family of receptor tyrosine kinases (a.k.a. ERBB receptors) mediate the effects of the epidermal growth factor (EGF) family including Neuregulin-1β (NRG). NRG and ERBB1-4 are critical for cardiac development and maintenance of the adult heart. Current understanding of the role of EGF/NRG/ERBB signaling in the cardiovascular system is rapidly evolving due to recent findings in non-myocyte cell populations. This study is examining a population of progenitor cells in the adult human heart that responds to EGF and NRG. Subjects are enrolled who are scheduled to undergo heart surgery and are willing to allow for a small biopsy to be taken from their hearts during surgery. Biopsies are taken to the laboratory where cells are separated and analyzed by flow cytometry and grown in cell culture to understand how their biology is regulated by NRG and EGF.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Clinical diagnosis of severe coronary artery disease scheduled to undergo coronary artery bypass surgery.
排除标准
- •less than 18 years of age
- •unwilling or unable to provide informed consent
- •known active myocarditis
- •hypertrophic cardiomyopathy
- •constrictive pericarditis or other significant pericardial disease
- •severe pulmonary hypertension
- •significant renal impairment (Cr > 2.5 mg/dL)
- •severe ventricular arrhythmias
- •pregnancy
结局指标
主要结局
The number of highly proliferative clones isolated
时间窗: up to 14 days from isolation
次要结局
- Flow cytometric ERBB receptor expression in highly proliferative clones isolated from heart tissue(up to 14 days from isolation)
研究者
Douglas B. Sawyer
Chief Academic Officer
MaineHealth