Phenotypic and Genotypic Characteristics of Pseudomonas Aeruginosa Isolates in Sohag University Hospitals
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Detection of biofilm forming isolates
研究概览
简要总结
Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 1 Year 至 80 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.
排除标准
- •Any infection before 48 hours of patient admission at Hospital
结局指标
主要结局
Detection of biofilm forming isolates
时间窗: 6 months
Detection of biofilm forming isolates by tissue culture plate method.
Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas
时间窗: 6 months
Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines
Isolation and identification of pseudomonas aeruginosa causing different nosocomial infections.
时间窗: 6 months
Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions
Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa.
时间窗: 6 months
Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction
次要结局
未报告次要终点
研究者
Martina Gamil Fayez
principle investigator
Sohag University