Health Effects of Salmon Fish Meal - ex Vivo Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 5
- 试验地点
- 1
- 主要终点
- Changes in the whole genome transciptome in cultured cells
研究概览
简要总结
Diabetes contributes significantly to the burden of disease in Norway and cardiovascular disease is the main cause of mortality.
Both lean and fatty fish are shown to have beneficial health effects. In addition to omega-3 fatty acids, fish contain potential health-promoting components such as taurine, vitamin D, vitamin B12, iodine, selenium and more unspecified components such as bioactive peptides. With the expected growth in the aquaculture sector, more protein-rich by-products will become available.
The overall aim of this project is to investigate physiological and molecular effects of fish protein in the form of salmon fishmeal compared to whey in a human intervention study with regard to carbohydrate- and fat metabolism, endocrine factors and inflammation.
The investigators will include healthy subjects to a randomized controlled cross over study. The subjects will receive a single, oral dose of fishmeal or whey. Blood samples are taken before (fasting) and 30 and 60 minutes after intake. The molecular effects of fishmeal and whey are investigated ex vivo. This is done by incubating human cell lines (i.e hepatocytes, adipocytes, epithelial cells) with fasting and postprandial serum from the participants.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Body mass index 18.5-24.9 kg/m2
排除标准
- •Body mass index <18.5 or >24.9 kg/m2
- •Known diabetes or high blood pressure
- •Pregnancy or breast feeding
- •Allergy or intolerance to dairy or fish/fish protein.
结局指标
主要结局
Changes in the whole genome transciptome in cultured cells
时间窗: Acute changes from baseline to 30 and/or 60 minutes.
Changes in the whole genome transciptome of cultured cells incubated with fasting and postprandial serum from participants receiving whey or fishmeal.
次要结局
- Changes in cytokine secretion in cultured cell lines(Acute changes from baseline to 30 and/or 60 minutes.)
- Changes in insulin-stimulated glucose uptake in cultured cell lines(Acute changes from baseline to 30 and/or 60 minutes.)
- Blood concentration of glucose(Acute changes from baseline to 30 and 60 minutes)
- Blood concentration of triglycerides(Acute changes from baseline to 30 and 60 minutes)
- Blood concentration of insulin(Acute changes from baseline to 30 and 60 minutes)
- Changes in histone marks on selected target genes in cultured cell lines(Acute changes from baseline to 30 and/or 60 minutes.)
- Markers in blood related to appetite(Acute changes from baseline to 30 and 60 minutes)
- Markers in blood related to low grade inflammation(Acute changes from baseline to 30 and 60 minutes)
- Markers in blood related to digestion of protein(Acute changes from baseline to 30 and/or 60 minutes)
- Blood concentration of cholesterol(Acute changes from baseline to 30 and 60 minutes)
研究者
Kirsten Holven
Professor
University of Oslo