The Use of Advanced Platelet-rich Fibrin in Mandibular Third Molar Surgery. Levels of Platelets, Leucocytes, Inflammatory Biomarkers and the Effect on Postoperative Discomfort and Complications.

Three venous whole blood samples will be collected by a dental assistant, which has undergone special training, supervised by a medical doctor affiliated with Specialtandlægerne Vestergade.

Surgical removal of M3 will be performed by one certified maxillofacial surgeon under local anaesthesia using a standard technique.

An incision from the anterior border of the ascending ramus of the mandible to the distal part of the lower first molar will be performed. The muco-periosteal flap will be reflected and the bone around the M3 will be removed with a round burr under constant irrigation with 0.9% saline solution. If necessary, the M3 will be sectioned with a fissure bur before the tooth is elevated out. The extraction socket and surrounding bone will be irrigated with 0.9 % saline solution, and the surgical site will be sutured with vicryl suture.

All patients will receive standard postoperative instructions. Sutures will be removed 7 days postoperatively. All patients will be seen in the clinic before surgery (T0) and one week following surgical removal of M3 .