The Effect of Short-term Overconsumption of Specific Dietary Nutrients on Liver and Adipose Tissue Metabolism
试验速览
- 阶段
- 不适用
- 入组人数
- 100
- 试验地点
- 2
- 主要终点
- Liver de novo lipogenesis
研究概览
简要总结
In health,adipose tissue stores fat from the diet. If the fat tissue stops storing dietary fat then this increases the chance of it being stored in the liver, which is related to increased risk of heart disease and type 2 diabetes. It has been suggested that having a diet high in sugars or certain fats, may cause the fat tissue and liver to change how they function; we would like to investigate this in detail.
详细描述
This research aims to investigate how the overconsumption of specific macronutrients may effect adipose tissue and liver metabolism and function. Liver and heart fat content and adipose tissue and liver metabolism fasting and postprandial lipid metabolism will be assessed before and after the specific dietary intervention.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Participant is willing and able to give informed consent for participation in the study.
- •Male or Female, aged ≥18 or ≤65 years.
- •Body Mass Index ≥19 ≤35 kg/m2
- •No medical condition or relevant drug therapy known to affect liver or adipose tissue metabolism.
- •Weight stable for the previous 3 months
排除标准
- •Patient is unwilling or unable to give informed consent for participation in the study
- •A blood haemoglobin <135mg/dL for men and <120mg/dL for women
- •Donated (or lost) ≥250 ml of blood in the previous two months.
- •On a weight loss diet or have decreased their body weight by >5% in the previous 3 months.
- •Have increased their body weight by >5% in the previous 3 months.
- •Any metabolic condition or relevant drug therapy
- •Current smoker
- •History of alcoholism or a greater than recommended alcohol intake (>30 g of alcohol daily for men and >20 g of alcohol daily for women)
- •Haemorrhagic disorders
- •Anticoagulant treatment
- •History of albumin allergy
- •Pregnant or nursing mothers
- •Women who are taking any contraceptive agent or device including oral contraceptives, hormone replacement therapy (HRT) or who have used these within the last 12 months
- •History of severe claustrophobia
- •Presence of metallic implants, pacemaker, or are unwilling to remove any piercings
- •History of an eating disorder or any other psychological condition that may affect the participants ability to adhere to study intervention/experimental diets.
结局指标
主要结局
Liver de novo lipogenesis
时间窗: Within 21 days after starting the intervention diet
Investigators will utilise stable isotope tracer methodology to measure the change in the contribution of newly synthesised fatty acids to very low density lipoprotein triglyceride
次要结局
- Liver fat accumulation(Within 21 days after starting the intervention diet)
- Adipose tissue metabolism(Within 21 days after starting the intervention diet)