In Vitro Biofilm Formation and Antibiotic Susceptibility Patterns of Bacteria From Surgical Site Infection After Orthopedic Surgery
试验速览
- 阶段
- 不适用
- 入组人数
- 100
- 主要终点
- detect biofilm formation
研究概览
简要总结
Detection of bacteria that form biofilm and its sensitivity to antibiotics in patients with surgical site infection after orthopedic surgery.
详细描述
The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to the following steps to identify the causative bacteria:
- Culturing on blood agar, chocolate agar, MacConkey agar and Mannitol salt agar , gram staining and biochemical tests.
- Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.
- Antimicrobial Susceptibility Testing using the Kirby-Bauer disk diffusion method.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •patients with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...)
排除标准
- •if patient has no orthopedic surgery
结局指标
主要结局
detect biofilm formation
时间窗: one year
The available number of patients presenting in outpatient clinic or at operating theatre with surgical site infection after orthopedic surgery that include insertion of implant (e.g plates , screws ,wires ,...) will be subjected to full history and examined by physician. Swabs from site of infection will be taken and subjected to Enzyme-linked immunosorbent assay (ELISA) to detect biofilm formation.
次要结局
未报告次要终点
研究者
Amira Ahmed Talaat
Principal investigator
Sohag University