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Soy and Isoflavones Effect on Bone

Phase 4
Completed
Conditions
Osteoporosis
Interventions
Dietary Supplement: Soy Isolate
Dietary Supplement: Control protein
Dietary Supplement: Novasoy isoflavones
Dietary Supplement: Placebo tablets
Registration Number
NCT00668447
Lead Sponsor
United States Department of Agriculture (USDA)
Brief Summary

The purpose of this study is to test the effect of 1 year of added dietary soy protein and/or soy isoflavones on bone mineral density in late postmenopausal women.

Detailed Description

Although soy foods contain several components (isoflavones and amino acids) that could potentially have positive effects on bone health, there are few long term, large, clinical trials using soy as a means of improving bone mineral density. The objective of this study is to provide daily soy protein and isoflavones to healthy older women in order to answer three major questions:

1. Does soy protein alone affect bone metabolism?

2. Do isoflavones, given with soy protein, affect bone metabolism?

3. What dose of isoflavones affects bone in older women?

We hypothesize that soy protein will have a beneficial effect on bone in older women compared to control protein. Further, we hypothesize that there will be an additional benefit to bone in women who receive soy protein plus isoflavones (at both doses) compared to soy protein alone.

Both control and soy proteins used in the study were isolates, meaning they were the highest concentration of protein (85-90% by weight) in order to minimize the volume of protein supplement that each woman was asked to ingest on a daily basis. The soy protein was an alcohol-washed, soy protein isolate containing 90% protein and negligible isoflavone (0.2 mg/g product). The control protein was a mix consisting of 50% protein from sodium caseinate, 25% protein from whey protein and 25% from egg white protein. The use of a mix of proteins as a control provides a more balanced level of amino acids, mimics the real life mix of proteins that humans typically consume, and avoids the unique characteristics of one source of protein. In order to maintain the dietary protein intake constant, the participant was counseled to decrease her intake of other sources of protein from primarily animal sources by approximately 3 ounces per day (the approximate equivalent of the protein powders). The isoflavones tablets each contained 57 mg of total isoflavone from primarily genistein, glycitein, and daidzein and their beta-glycosides.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
97
Inclusion Criteria
  • Postmenopausal women 65 years old or older
  • Able to travel to the clinical sites for follow-up visits
Exclusion Criteria
  • History of disease that may affect bone metabolism (including Paget's disease, primary hyperparathyroidism, osteomalacia, untreated hyperthyroidism, or multiple myeloma)
  • Cancer of any kind (except basal or squamous cell of skin) in past 5 years
  • Use of any of the following medications within the past 2 years: calcitonin, calcitriol, heparin, phenytoin, phenobarbital
  • Use at any time of bisphosphonates, long-term corticosteroids (over 6 months), methotrexate, or fluoride
  • Estimated creatinine clearance less than 50 ml/min
  • History of chronic liver disease or evidence of liver disease on screening
  • History of hip fracture
  • Known vertebral fracture within the past year
  • Vegans

Study & Design

Study Type
INTERVENTIONAL
Study Design
FACTORIAL
Arm && Interventions
GroupInterventionDescription
1Soy IsolateSoy protein and isoflavone tablets
1Novasoy isoflavonesSoy protein and isoflavone tablets
2Soy IsolateSoy protein and placebo tablets
2Placebo tabletsSoy protein and placebo tablets
3Control proteincontrol protein and Isoflavone tablets
3Novasoy isoflavonescontrol protein and Isoflavone tablets
4Control proteincontrol protein and placebo tablets
4Placebo tabletscontrol protein and placebo tablets
Primary Outcome Measures
NameTimeMethod
Bone turnover markersbaseline, 3 months, and 1 year
Secondary Outcome Measures
NameTimeMethod
Bone Mineral DensityBaseline, 6 and 12 months
Quality of life measured by Medical Outcomes Short FormBaseline, 6 and 12 months
Medication Side Effects3 , 6, 9, and 12 months
Adherence to dietary intervention through the use of 24-hour recall3, 6, 9, and 12 months
Long-term medication behavior self-efficacy scale3, 6, 9, and 12 months

Trial Locations

Locations (1)

University of Connecticut Health Center

🇺🇸

Farmington, Connecticut, United States

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