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CD8+ T Cell Therapy and Pembrolizumab in Treating Patients With Metastatic Gastrointestinal Tumors

Phase 1
Terminated
Conditions
Colorectal Adenocarcinoma
Metastatic Gastric Carcinoma
Metastatic Cholangiocarcinoma
Metastatic Esophageal Carcinoma
Metastatic Pancreatic Adenocarcinoma
Stage IV Gastric Cancer AJCC v7
Stage IV Pancreatic Cancer AJCC v6 and v7
Stage IV Esophageal Cancer AJCC v7
Stage IVA Colorectal Cancer AJCC v7
Metastatic Colorectal Carcinoma
Interventions
Biological: Adoptive Immunotherapy
Biological: Aldesleukin
Drug: Cyclophosphamide
Other: Laboratory Biomarker Analysis
Biological: Pembrolizumab
Registration Number
NCT02757391
Lead Sponsor
M.D. Anderson Cancer Center
Brief Summary

This phase I pilot trial studies the side effects of cluster of differentiation 8 (CD8)+ T cells in treating patients with gastrointestinal tumors that have spread to other places in the body. Tumor cells and blood are used to help create an adoptive T cell therapy, such as CD8+ T cell therapy, that is individually designed for a patient and may help doctors learn more about genetic changes in the tumor. Immunotherapy with monoclonal antibodies, such as pembrolizumab, may help the body's immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread. Giving CD8+ T cell therapy and pembrolizumab may work better in treating patients with gastrointestinal tumors.

Detailed Description

PRIMARY OBJECTIVES:

I. Assess the safety of using a personalized adoptive T cell therapy in patients with advanced gastrointestinal malignancies.

SECONDARY OBJECTIVES:

I. Assess the persistence of an immune response after T cell infusion. II. Determine the clinical benefit of adoptive T cell therapy in advanced gastrointestinal cancers.

OUTLINE:

Beginning 2 days prior to CD8+ T cell infusion, patients receive cyclophosphamide intravenously (IV) over 30 minutes. Patients undergo CD8+ T cell infusion IV over 2 hours on day 0 and receive aldesleukin subcutaneously (SC) twice daily (BID) on days 1-14. Beginning on day 1 about 24 hours after CD8+ T cell infusion, patients receive pembrolizumab IV over 30-60 minutes on weeks 3, 6, 12, and 15.

After completion of study treatment, patients are followed up for 24 weeks.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
1
Inclusion Criteria

Not provided

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Exclusion Criteria

Not provided

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Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Treatment (CD8 +T cell therapy, pembrolizumab)Laboratory Biomarker AnalysisBeginning 2 days prior to CD8+ T cell infusion, patients receive cyclophosphamide IV over 30 minutes. Patients undergo CD8+ T cell infusion IV over 2 hours on day 0 and receive aldesleukin SC BID on days 1-14. Beginning on day 1 about 24 hours after CD8+ T cell infusion, patients receive pembrolizumab IV over 30-60 minutes on weeks 3, 6, 12, and 15.
Treatment (CD8 +T cell therapy, pembrolizumab)AldesleukinBeginning 2 days prior to CD8+ T cell infusion, patients receive cyclophosphamide IV over 30 minutes. Patients undergo CD8+ T cell infusion IV over 2 hours on day 0 and receive aldesleukin SC BID on days 1-14. Beginning on day 1 about 24 hours after CD8+ T cell infusion, patients receive pembrolizumab IV over 30-60 minutes on weeks 3, 6, 12, and 15.
Treatment (CD8 +T cell therapy, pembrolizumab)Adoptive ImmunotherapyBeginning 2 days prior to CD8+ T cell infusion, patients receive cyclophosphamide IV over 30 minutes. Patients undergo CD8+ T cell infusion IV over 2 hours on day 0 and receive aldesleukin SC BID on days 1-14. Beginning on day 1 about 24 hours after CD8+ T cell infusion, patients receive pembrolizumab IV over 30-60 minutes on weeks 3, 6, 12, and 15.
Treatment (CD8 +T cell therapy, pembrolizumab)CyclophosphamideBeginning 2 days prior to CD8+ T cell infusion, patients receive cyclophosphamide IV over 30 minutes. Patients undergo CD8+ T cell infusion IV over 2 hours on day 0 and receive aldesleukin SC BID on days 1-14. Beginning on day 1 about 24 hours after CD8+ T cell infusion, patients receive pembrolizumab IV over 30-60 minutes on weeks 3, 6, 12, and 15.
Treatment (CD8 +T cell therapy, pembrolizumab)PembrolizumabBeginning 2 days prior to CD8+ T cell infusion, patients receive cyclophosphamide IV over 30 minutes. Patients undergo CD8+ T cell infusion IV over 2 hours on day 0 and receive aldesleukin SC BID on days 1-14. Beginning on day 1 about 24 hours after CD8+ T cell infusion, patients receive pembrolizumab IV over 30-60 minutes on weeks 3, 6, 12, and 15.
Primary Outcome Measures
NameTimeMethod
Incidence of toxicity defined as grade 3 or 4 non-hematologic or grade 4 hematologic toxicity per Common Terminology Criteria for Adverse Events version 4.0Up to 24 weeks

Will monitor toxicity of the personalized vaccines in using cohorts of size of 5, starting from the 1st patient using the Bayesian approach of Thall, Simon, Estey.

Secondary Outcome Measures
NameTimeMethod
Persistence of an immune response defined by T cell interferon gamma release in response to selected personalized peptide antigensUp to 24 weeks
Persistence of an immune response defined by levels of intracellular cytokine staining of T cells in response to stimulation with personalized peptide antigensUp to 24 weeks
Persistence of an immune response defined by detection of antigen spreadingUp to 24 weeks
Time to progressionUp to 24 weeks
Persistence of an immune response defined by level of tetramer positive T cell population over time after T cell infusionUp to 24 weeks
Proportion of patients who have received T cell infusion that is alive and progression free (complete response [CR] + partial response [PR] + stable disease) defined based on response criteria according to Response Evaluation Criteria in Solid Tumors 1.1At 12 weeks post infusion
Response rate (CR + PR)Up to 24 weeks

The response rate will be evaluated using a Simon optimal two-stage design.

Overall survivalUp to 24 weeks

Trial Locations

Locations (1)

M D Anderson Cancer Center

🇺🇸

Houston, Texas, United States

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