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Neoadjuvant L19IL2/L19TNF- Pivotal Study

Phase 3
Recruiting
Conditions
Malignant Melanoma
Interventions
Procedure: Surgery
Registration Number
NCT02938299
Lead Sponsor
Philogen S.p.A.
Brief Summary

Phase III, open-label, randomized, controlled multi-center study of the efficacy of L19IL2/L19TNF neoadjuvant intratumoral treatment in Stage III B/C melanoma patients.

Detailed Description

Phase III, open-label, randomized, controlled multi-center study. In the study, 214 patients will be enrolled and parallel assigned (via automated randomization system) in a 1:1 fashion to one of two different arms:

ARM 1:

Patients in Arm 1 will receive multiple intratumoral administrations into all injectable cutaneous, subcutaneous, and nodal tumors of a mixture of L19IL2 and L19TNF once weekly for up to 4 weeks (or until all injectable tumors have disappeared, or intolerance to study treatment or in the opinion of the investigator immediate surgical resection or any other treatment for melanoma is warranted, whichever occurs first). The whole volume of L19IL2/L19TNF will be distributed among all injectable lesions.

Newly occurring injectable melanoma lesions within the 4 weeks treatment period will also be treated as described. For the new lesions the treatment period will not be extended beyond the pre-defined 4 week- treatment period with a clock start at the time of the first intralesional L19IL2/L19TNF injection. Surgical resection of all existing metastases will follow within 4 weeks after end of treatment. Surgery will be performed after the safety evaluation carried out at week 5 and, if indicated, may be carried out on the same day of the safety evaluation.

Post-surgery EMA-approved adjuvant therapy is allowed at discretion of the treating physician.

ARM 2:

Patients in Arm 2 will receive directly surgical resection of melanoma tumor lesions within 4 weeks after randomization.

Post-surgery EMA-approved adjuvant therapy is allowed at discretion of the treating physician.

Patients will be followed on a regular basis for the primary outcome until 36 months from randomization and up to 60 months for overall survival.

Expected patient enrollment interval: 60 months. Duration of individual patient's participation: up to 60 months. End of treatment corresponds to the day of surgery for patients randomized to both Arm 1 and Arm 2.

End of study corresponds to the last patient last visit (LPLV). The final primary efficacy analysis will be conducted when the 95th recurrence event is observed.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
214
Inclusion Criteria

Not provided

Exclusion Criteria
  1. Uveal melanoma, mucosal melanoma or melanoma with unknown primary.
  2. Evidence of distant metastases at screening.
  3. Previous or concurrent cancer that is distinct in primary site or histology from the cancer being evaluated in this study except cervical carcinoma in situ, treated basal cell carcinoma, superficial bladder tumors (Ta, Tis & T1), second primary melanoma in situ or any cancer curatively treated ≥ 5 years prior to study entry.
  4. Presence of active infections (e.g., requiring antimicrobial therapy) or other severe concurrent disease, which, in the opinion of the investigator, would place the patient at undue risk or interfere with the study.
  5. History within the last year of acute or subacute coronary syndromes including myocardial infarction, unstable or severe stable angina pectoris.
  6. Inadequately controlled cardiac arrhythmias including atrial fibrillation.
  7. Heart insufficiency (> Grade II, New York Heart Association (NYHA) criteria).
  8. LVEF ≤ 50% and/or abnormalities observed during baseline ECG and Echocardiogram investigations that are considered as clinically significant by the investigator.
  9. Uncontrolled hypertension.
  10. Ischemic peripheral vascular disease (Grade IIb-IV).
  11. Severe diabetic retinopathy.
  12. Active autoimmune disease.
  13. History of organ allograft or stem cell transplantation.
  14. Recovery from major trauma including surgery within 4 weeks prior to enrollment.
  15. Known history of allergy to IL2, TNF, or other human proteins/peptides/antibodies or any other constituent of the product.
  16. Breast feeding female.
  17. Anti-tumor therapy (except allowed treatments listed at point 3 of Inclusion criteria) within 4 weeks before enrollment.
  18. Previous in vivo exposure to monoclonal antibodies for biological therapy (except allowed treatments listed at point 3 of Inclusion criteria) in the 6 weeks before enrollment.
  19. Planned administration of growth factors or immunomodulatory agents (except allowed treatments listed at point 3 of Inclusion criteria) within 7 days before enrollment.
  20. Patient requiring or taking corticosteroids or other immunosuppressant drugs on a long-term basis. Limited use of corticosteroids to treat or prevent acute hypersensitivity reactions is not considered an exclusion criterion.
  21. Any conditions that in the opinion of the investigator could hamper compliance with the study protocol.
  22. Previous enrolment and randomization in this same study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Arm 1: neoadjuvant + surgeryL19IL2 + L19TNFPatients in Arm 1 will receive multiple intratumoral administrations into all injectable cutaneous, subcutaneous, and nodal tumors of a mixture of L19IL2 and L19TNF once weekly for up to 4 weeks (or until all injectable tumors have disappeared, or intolerance to study treatment or in the opinion of the investigator immediate surgical resection or any other treatment for melanoma is warranted, whichever occurs first). Newly occurring injectable melanoma lesions within the 4 weeks treatment period will also be treated as described. Surgical resection of all existing metastases will follow within 4 weeks after end of treatment. Surgery will be performed after the safety evaluation carried out at week 5 and, if indicated, may be carried out on the same day of the safety evaluation.
Arm 1: neoadjuvant + surgerySurgeryPatients in Arm 1 will receive multiple intratumoral administrations into all injectable cutaneous, subcutaneous, and nodal tumors of a mixture of L19IL2 and L19TNF once weekly for up to 4 weeks (or until all injectable tumors have disappeared, or intolerance to study treatment or in the opinion of the investigator immediate surgical resection or any other treatment for melanoma is warranted, whichever occurs first). Newly occurring injectable melanoma lesions within the 4 weeks treatment period will also be treated as described. Surgical resection of all existing metastases will follow within 4 weeks after end of treatment. Surgery will be performed after the safety evaluation carried out at week 5 and, if indicated, may be carried out on the same day of the safety evaluation.
Arm 2: surgery aloneSurgeryPatients in Arm 2 will receive directly surgical resection of melanoma tumor lesions within 4 weeks after randomization.
Primary Outcome Measures
NameTimeMethod
Recurrence-free survival (RFS) rate1 year after randomization

Recurrence-free survival (RFS) in the treatment arm (L19IL2/L19TNF plus surgery; Arm 1) versus control arm (Arm 2).

Secondary Outcome Measures
NameTimeMethod
Overall survival (OS)1year, 2years, 3years after randomization
Clinically Meaningful Changes in Vital Signs and Physical Examinationsup to 36 months
Recurrence-free survival (RFS) rate2years, 3years after randomization
Percentage of Participants With Worst On-Study Hematological and Chemistry Abnormalitiesup to 36 months
HAFA(1) At Day 1, (2) At Day 29, (3) After 3 months from surgery: Day 119 to Day 144

Assessment of the formation of human anti-fusion protein antibodies (HAFA) against L19IL2 and L19TNF.

Local recurrence-free survival (LRFS)1year, 2years, 3years after randomization and 1year after surgery
Distant metastasis-free survival (DMFS) rate1year, 2years, 3years after randomization and 1year after surgery
Percentage of Participants With On-Study Adverse Events (AEs) and Serious Adverse Events (SAEs)up to 3 years
Changes in absolute counts and relative percentages of lymphocytic subpopulations over time(1) At screening, (2) At Day of surgery: from Day 1 to Day 54, (3) After 3 months from surgery: Day 91 to Day 144

Immunophenotypic characterization of PBMCs for changes in absolute counts and relative percentages of lymphocytic subpopulations (e.g., Tregs, MDSCs etc.) over time (only for patients recruited in German centers).

Trial Locations

Locations (21)

Hôpital de la Timone

🇫🇷

Marseille, France

Hôpital Universitaire de Nantes

🇫🇷

Nantes, France

Institut Gustave Roussy

🇫🇷

Villejuif, France

Klinik für Dermatologie und Allergologie, Universitätsklinikum Augsburg

🇩🇪

Augsburg, Germany

Charité Campus Mitte (CCM)

🇩🇪

Berlin, Germany

Universitätsklinikum Carl Gustav Carus an der Technischen Universität Dresden

🇩🇪

Dresden, Germany

Klinik für Dermatologie, Medizinische Fakultät Universitätsklinikum Essen

🇩🇪

Essen, Germany

Hauttumorzentrum Hannover (HTZH)

🇩🇪

Hannover, Germany

Heidelberg University Hospital

🇩🇪

Heidelberg, Germany

Kiel University Hospital

🇩🇪

Kiel, Germany

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Hôpital de la Timone
🇫🇷Marseille, France

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