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Intramyocardial Injection of Autologous Umbilical Cord Blood Derived Mononuclear Cells During Surgical Repair of Hypoplastic Left Heart Syndrome

Phase 2
Active, not recruiting
Conditions
Hypoplastic Left Heart Syndrome
Interventions
Biological: Autologous (self) mononuclear cells derived from umbilical cord blood
Procedure: Stage II Surgical repair
Registration Number
NCT03779711
Lead Sponsor
Timothy J Nelson, MD, PhD
Brief Summary

Researchers want to better understand what happens to the heart when the stem cells are injected directly into the muscle of the right side of the heart during the Stage II palliative surgery for single ventricle patients with hypoplastic left heart syndrome (HLHS) or HLHS variant. Researchers want to see if there are changes in the heart's structure/function following this stem cell-based therapy and compared to children that have not had cell-based therapy.

Detailed Description

This is a Phase IIb trial to determine if the delivery of the autologous UCB-MNC product into the myocardium of the right ventricle of the heart at the time of Stage II surgical repair will provide an improvement in cardiac function, reaching growth and developmental milestones, and quality of life, while also providing a reduction in the cumulative days of hospitalization following Stage II surgical repair. Long-term improvement in cardiac function, reaching growth and developmental milestones, reaching Stage III surgical repair pre-op work-up, prolonging time to cardiac transplantation or death, and improving quality of life will also be determined.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
95
Inclusion Criteria
  • Diagnosis of HLHS or HLHS variant with single right ventricular dependent CHD having undergone Stage I surgical repair and undergoing Stage II surgical repair.
  • Less than 13 months of age at time of Stage II surgical repair
  • Previous participation in the UCB collection protocol with autologous UCB-MNC product that is acceptable for use (treatment arm only)
Exclusion Criteria
  • History of DMSO reaction (treatment arm only).

  • Parent(s) and/or legal guardian(s) unwilling to have their child participate or unwilling to follow the study procedures.

  • Severe chronic diseases at the discretion of the treating physician.

  • Extensive extra-cardiac syndromic features.

  • Known history of cancer.

  • Any of the following complications of his/her congenital heart disease:

    1. Any condition requiring urgent, or unplanned interventional procedure within 15 days prior to Stage II surgical repair, unless complete and full cardiac recovery is documented by site investigator
    2. Severe pulmonary hypertension (reported in the medical record as >70% systemic pressure)
    3. Other clinical concerns as documented by a site investigator that would predict (more likely to happen than not to happen) a risk of severe complications or very poor outcome, not directly related to the stem cell product or its injection procedure, during or after Stage II surgical repair.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
TreatmentAutologous (self) mononuclear cells derived from umbilical cord bloodAutologous (self) mononuclear cells derived from umbilical cord blood and that meet all release criteria are injected into the surface of the right heart muscle to achieve the target dose of 1-3 million cells per kilogram body weight . This is a one time treatment at the time of Stage II Glenn surgery .
TreatmentStage II Surgical repairAutologous (self) mononuclear cells derived from umbilical cord blood and that meet all release criteria are injected into the surface of the right heart muscle to achieve the target dose of 1-3 million cells per kilogram body weight . This is a one time treatment at the time of Stage II Glenn surgery .
ControlStage II Surgical repairClinical data will be collected before, during and after the Stage II surgical standard of care procedure. Requires additional imaging studies at 3 months that are not standard of care in addition to some blood tests that would be beyond standard of care. Information will be collected to document the clinical outcomes and will be compared with the information from the group receiving the investigational treatment used in this study.
Primary Outcome Measures
NameTimeMethod
Change in Right Ventricular Cardiac Function Measured by Apical Fractional Area Change (FAC)baseline, 12 months post-Stage II surgery

Right ventricular cardiac function as measured by echocardiogram that includes studies for apical fractional area change (FAC). Measured by percent.

Change in Right Ventricular Cardiac Function Measured by Circumferential Strain.baseline, 3 months post-Stage II surgery

Right ventricular cardiac function as measured by echocardiogram that includes studies for circumferential strain. Measured by percent.

Change in Right Ventricular Cardiac Function Measured by Longitudinal Strain.baseline, 3 months post-Stage II surgery

Right ventricular cardiac function as measured by echocardiogram that includes studies for longitudinal strain. Measured by percent.

Secondary Outcome Measures
NameTimeMethod
Change in Right Ventricular Cardiac Function Measured by Apical Fractional Area Change (FAC)baseline, Stage III surgery pre-op

Right ventricular cardiac function as measured by echocardiogram that includes studies for apical fractional area change (FAC). Measured by percent.

Cumulative Days of Hospitalization3-months post Stage II surgery

Cumulative days of hospitalization per patient following discharge for the Stage II surgical repair.

Change in Weightbaseline, every 6 months post-Stage II surgery

Weight as measured by scale provided as part of standard of care by subject's provider. Measured at home by subject's caregiver using the same scale for every home measurement of body weight. Caregivers will be instructed by the site to use the same scale for every home measurement of body weight. Scales will vary based on the institution's standard name/type used.

Change in Heart Ratebaseline, 3-months post Stage II surgery

Change in heart rate between baseline and 3-months post Stage II surgery. Heart rate as measured at home by subject's caregiver as standard of care per subject's provider. Caregivers will be instructed by the site staff on the best way to perform.

Change in Oxygen Saturationbaseline, 3-months post Stage II surgery

Change in oxygen saturation between baseline and 3-months post Stage II surgery. Oxygen saturation as measured at home by subject's caregiver using the pulse oximetry device provided as standard of care by subject's provider. Caregivers will be instructed by the site to use the same pulse oximetry device for every home measurement of oxygen saturation.

Change in Arrhythmia and Heart Failure Medication3-months post-surgery

Changes in arrhythmia and heart failure medications will be recorded by caregiver on the concomitant medication diary throughout the subject's participation in the study starting at the time of consent.

Stage III Surgical RepairStage III surgery pre-op, approx. 4 years

The number of research subjects eligible for Stage III surgical repair

Total Time Until Listed for Cardiac Transplantationup to Stage III surgery pre-op, approx. 4 years

The number of days between Stage II surgery and listed on cardiac transplantation list.

Total Time Until Deathapprox 4 years

The number of days between Stage II surgery and death occurrence

Trial Locations

Locations (8)

Children's of Alabama

🇺🇸

Birmingham, Alabama, United States

Children's Hospital of Los Angeles

🇺🇸

Los Angeles, California, United States

Children's Hospital Colorado

🇺🇸

Aurora, Colorado, United States

Ocshner Medical Center

🇺🇸

New Orleans, Louisiana, United States

Children's Hospitals of Minnesota

🇺🇸

Minneapolis, Minnesota, United States

Cincinnati Children's Hospital and Medical Center

🇺🇸

Cincinnati, Ohio, United States

Oklahoma University Medical Center

🇺🇸

Oklahoma City, Oklahoma, United States

Children's Hospital of Philadelphia

🇺🇸

Philadelphia, Pennsylvania, United States

Children's of Alabama
🇺🇸Birmingham, Alabama, United States
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