Estimation and comparison of salivary cytokines and alpha amylase in whole saliva of children with type 1 Diabetes Mellitus and Non Diabetic children
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- To estimate and compare the levels of salivary cytokines using ELISA .To estimate and compare the levels of alpha amylase using UV spectrometer in diabetic and non-diabetic children
研究概览
简要总结
The present study will be done on 40 subjects including 20 children with confirmed type 1 diabetes and 20 diabetes free children aged 7-15 years to estimate and compare the levels of salivary cytokines and alpha amylase in whole saliva among type 1 diabetic children and non-diabetic children an unstimulated salivary samples is collected by spitting method The patients will be asked to rinse mouth with filtered water twice before the collection after the oral rinse the children will be asked to spit the saliva into sterile tubes for a period of 5 mins The collected volume of saliva will be measured in millimeters per minute to estimate flow rate the tubes will then be sealed immediately after sample collection and refrigerated at 4℃ before transporting to laboratory for further analysis
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 7.00 Year(s) 至 15.00 Year(s)（—）
- 性别
- All
入选标准
- •1Children of 7-15 years of age.
- •2Children with insulin dependent type 1 diabetes mellitus disorder.
- •3Parents giving consent for the study.
排除标准
- •1 Children with presence of any physical or mental disorder.
- •2 Children requiring special health care needs.
- •3 Children with genetic diseases or syndromes.
- •4 Children on any other systemic medications.
结局指标
主要结局
To estimate and compare the levels of salivary cytokines using ELISA .To estimate and compare the levels of alpha amylase using UV spectrometer in diabetic and non-diabetic children
时间窗: baseline
次要结局
- estimating the values of salivary cytokines(baseline)
研究者
Dr Priyanka N
RajaRajeswari dental college and hospital