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Measurement of gut permeability in healthy people with a different metabolic profile.

Conditions
Metabolic health (waist circumference, BMI, fasting glucose, HbA1c, LDL cholesterol, HDL cholesterol, total cholesterol, total triglycerides, ALT, GGT and hsCRP)
Registration Number
NL-OMON20611
Lead Sponsor
Stichting Dienst Landbouwkundig OnderzoekWageningen UR Food & Biobased ResearchConsumer Science & HealthBornse Weilanden 96708 WG Wageningen
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Other
Sex
Not specified
Target Recruitment
30
Inclusion Criteria

Apparently healthy

-Age range between 20-70 years old (the two groups should be age balanced as much as possible)

Exclusion Criteria

-Gastrointestinal disorders (stomach ulcer, ulcerative colitis, Crohn¡¯s disease, celiac disease)

-History of gastrointestinal surgery

Study & Design

Study Type
Observational non invasive
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
The main study parameter is gut permeability assessed by a MS permeability test. In this test, gut permeability is reflected by the urinary excretion levels of sucrose, lactulose, rhamnose, sucralose and erythritol. These sugars are measured in 3 urine fractions that are collected during 24 hours after consumption of a MS mix solution containing these five sugars. Gut permeability will be compared between metabolic healthier and metabolic unhealthier subjects.
Secondary Outcome Measures
NameTimeMethod
Secondary parameters are fasting state levels of leaky gut biomarkers (LPS, LBP, sCD14, zonulin and leptin) and metabolic health parameters (waist, BMI, HbA1c, glucose, LDL cholesterol, HDL cholesterol, total cholesterol, total triglycerides, ALT, GGT and CRP). These parameters will be compared between metabolic healthier and metabolic unhealthier subjects and correlated with gut permeability as assessed by the MS permeability test.
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