The effect of red meat consumption on human metabolic and gut health in free living adults.

• Conditions: Metabolic Health; Gut microbiome community profiling; Metabolic and Endocrine - Normal metabolism and endocrine development and function; Oral and Gastrointestinal - Normal oral and gastrointestinal development and function

• Registration Number: ACTRN12619000896178
• Lead Sponsor: RMIT University

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2019-06-26
• Last Update Posted: 15 July 2019

Recruitment & Eligibility

• Status: ot yet recruiting
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

Healthy weight (body mass index 18.5 - 25kg/m2) males and females adults (aged 18 to 50) who are deemed eligible to participate as indicated by not meeting one or more of the exclusion criterion.

Exclusion Criteria

Participants will be excluded from participating within the project for the following reasons: Individuals with presence of chronic diseases related to the metabolism of energy and nutrients (i.e. hyperthyroidism, diagnosed diabetes), pathologic eating disorders, individuals with the presence of gastrointestinal disorders and diseases (i.e. inflammatory bowel disease, irritable bowel syndrome, coeliac disease, gastroenteritis), pregnant, menopausal/post-menopausal, taking contraindicated medication (i.e. psychotropic drugs, antibiotics, immunosuppressive drugs, or appetite suppressants), the use of any dietary/nutritional supplement that might interfere with the results of the study, weight unstable in the past 3 months, or irregular bowel patterns.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Changes in the gut microbiome composition (this is an exploratory outcome) <br><br>Characterisation of the changes to the gut microbial population dynamics in response to regulated red meat intake using metagenomics. Abundances of gut microbiota taxa, communities and microbiome diversity will be determined by 16S rRNA gene sequencing from stool samples.[Day 1, 8, 15, 22, 29];Changes in indicators of metabolic health (exploratory outcome)<br><br>Characterisation of the inter- and intra-individual changes to indicators of metabolic health in response to red meat intake using untargeted metabolomics <br><br><br>[Days 1, 8, 15, 22, 29];Changes in indicators of gut health (exploratory outcome)<br><br>Characterisation of the inter- and intra-individual changes to indicators of gut health in response to red meat intake using untargeted metabolomics <br>[Days 1, 8, 15, 22, 29]

Secondary Outcome Measures

Name	Time	Method
Changes in stool metabolite profile (exploratory outcome)<br><br>Characterisation of the inter- and intra-individual changes to stool metabolites in response to red meat intake using untargeted metabolomics <br><br><br> [Days 1, 8, 15, 22, 29];Changes in urine metabolite profile (exploratory outcome)<br><br>Characterisation of the inter- and intra-individual changes to urine metabolites in response to red meat intake using untargeted metabolomics <br>[Days 1, 8, 15, 22, 29]