Cartilage Adaptation and Response to Interleukins and Exercise
- Conditions
- Osteo Arthritis Knee
- Interventions
- Behavioral: Exercise.
- Registration Number
- NCT03410745
- Lead Sponsor
- Bispebjerg Hospital
- Brief Summary
This study will examine the effect of exercise on the turnover of collagen in articular cartilage and the content of cytokines in the synovial fluid from human adults with osteoarthritis.
- Detailed Description
By using stabile isotope labelling, this study will track the formation and destination of newly formed cartilage collagen in adult humans scheduled for knee replacement surgery due to osteoarthritis. The collagen formation as well as the effect on the cytokine content of the synovial fluid will be examined both at rest and in response to 4 weeks of exercise/training. Overall the project aims to improve the basic understanding of cartilage development and function in relation to osteoarthritis prevention.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 32
- Primary osteoarthritis of the knee scheduled for knee replacement surgery
- Must be able to perform physical exercise
- Age 40-90 years old
- BMI 18.5-40 kg/m^2
- None smoker for the last 6 weeks
- Regular intake of:
- non steroid anti-inflammatory drugs (NSAID)
- corticoid-steroids
- disease modifying anti-rheumatic drugs (DMARD)
- cytostatic incl. chemotherapy
- immune suppressive medicine
- Inflammatory diseases or cancer
- Recent trauma to the knee (< 3 months)
- Previous knee surgery
- Regular strenuous exercise ( > 1 pr. month)
- Previous exposure to tracers (e.g. from another study)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Exercise group Exercise. -
- Primary Outcome Measures
Name Time Method Collagen synthesis in articular cartilage End of study at 4 weeks By using incorporation of stabile isotope tracers measured by gas chromatography, the concentration of newly formed collagen will be measured.
- Secondary Outcome Measures
Name Time Method Age At the beginning of the study. The age measured in years
Cytokine and enzymes concentration in synovial fluid End of study at 4 weeks By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in synovial fluid will be measured.
Cytokine and enzymes concentration in blood At the beginning and at the end of the study at 4 weeks By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in the blood will be measured.
Gene expression End of study at 4 weeks By using real time reverse transcriptase polymerase chain reaction (RT-PCR) the concentration of messenger ribonucleic acid (mRNA) in cartilage tissue will be measured.
Strength At the beginning and at the end of the study at 4 weeks By calculating the total volume of weight lifted (kg) the strength will be measured.
Body mass index (BMI) At the beginning of the study. BMI measured as the total body weight divided by the height squared (kg/m\^2)
Trial Locations
- Locations (1)
Institute of Sports Medicine Copenhagen
🇩🇰Copenhagen, Denmark