Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities

Not Applicable

Completed

• Conditions: Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities; Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile; Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities
• Interventions: Behavioral: Low intensity exercise session; Behavioral: Moderate intensity exercise session; Behavioral: High intensity exercise session

• Registration Number: NCT05365334
• Lead Sponsor: University of Michigan

Brief Summary

The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.

Detailed Description

Not available

Study Timeline

• Study Start: 2021-12-13
• Study First Submitted: 2022-04-08
• Study First Submitted QC: 2022-05-03
• Study First Posted: 2022-05-09
• Primary Completion: 2023-06-23
• Study Completion: 2023-06-23
• Status Verified: 2024-07
• Last Update Submitted: 2024-07-25
• Last Update Posted: 2024-07-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 45

Inclusion Criteria

• Age:18-40
• Body Mass Index: 20-30 kg/m2
• Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
• Women must have regularly occurring menses and must be premenopausal
• Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository

Exclusion Criteria

• Evidence/history of cardiovascular or metabolic disease.
• Taking anti-coagulate medications or medications known to affect metabolism or inflammation
• Weight instability ≥ ±3kg in the last 6 months
• Tobacco or e-cigarette users
• Women must not be pregnant or actively lactating

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
low-intensity continuous exercise	Low intensity exercise session	subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
moderate-intensity continuous exercise	Moderate intensity exercise session	subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
high-intensity interval exercise	High intensity exercise session	subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between

Primary Outcome Measures

Name	Time	Method
mRNA expression of adipose tissue metabolic enzymes	75 minutes after exercise	qPCR
mRNA expression of skeletal muscle oxidative enzymes	60 minutes after exercise	qPCR
post-translational modifications of skeletal muscle oxidative enzymes	60 minutes after exercise	Western blot
post-translational modifications of Adipose tissue metabolic proteins	75 minutes after exercise	Western blot

Trial Locations

Locations (1)

University of Michigan; 🇺🇸 Ann Arbor, Michigan, United States