Exercise-induced Changes in Exosomes

Not Applicable

Completed

• Conditions: Exosomes; Exercise; Connective Tissue
• Interventions: Procedure: Resistance Exercise

• Registration Number: NCT05616234
• Lead Sponsor: University of California, Davis

Brief Summary

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Detailed Description

Not available

Study Timeline

• Study Start: 2022-11-07
• Study First Submitted: 2022-11-07
• Study First Submitted QC: 2022-11-07
• Study First Posted: 2022-11-15
• Primary Completion: 2023-02-17
• Study Completion: 2023-02-17
• Status Verified: 2023-06
• Last Update Submitted: 2023-06-13
• Last Update Posted: 2023-06-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• Young healthy adults (18-35 y)
• Experience with exercise (>1y)

Exclusion Criteria

• Pregnancy
• Smoking
• Receiving any medication that may interfere with the study outcomes

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Exercise trial	Resistance Exercise	Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn.

Primary Outcome Measures

Name	Time	Method
Collagen content of ligament constructs	15 min post-exercise	Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.

Secondary Outcome Measures

Name	Time	Method
Ultimate tensile stress and modulus of ligament constructs	15 min post-exercise	ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of ultimate tensile stress and modulus.
Exosomal RNA	15 min post-exercise	Isolation and sequencing of exosomal RNA from serum obtained at baseline or post-exercise.

Trial Locations

Locations (1)

Hickey Laboratory; 🇺🇸 Davis, California, United States