The role of muscular periarteriolar adipose tissue on vasoreactivity

Completed

• Conditions: Insulin-resistance; microvascular dysfunction; 10018424; 10057166

• Registration Number: NL-OMON37944
• Lead Sponsor: Vrije Universiteit Medisch Centrum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 6 May 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 50

Inclusion Criteria

-Age 18-55 years
-BMI >30 AND a waist-circumference of >94 cm in males, >80 cm in females, or BMI <25 AND a waist-circumference of <94 cm in males, <80 cm in females.

Exclusion Criteria

-Diabetes or impaired glucose tolerance (FPG > 6.1 mmol/l)
-Hypertension or other cardiovascular disease (stroke, coronary artery disease, peripheral vascular disease, heart failure) in medical history
-Use of medication for hypertension or diabetes, as well as use of medication known to disturb endothelial function or glucose metabolism
-Physical exercise (sporting) more than two times a week
-Pregnancy or intention to become pregnant during the study period
-Smoking

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The first main study parameter is the rate of muscle microvascular recruitment<br /><br>on insulin infusion, as measured by change of signal strength during contrast<br /><br>enhanced ultrasonography.<br /><br>The second main outcome is the amount of mPAT in muscle biopsies obtained from<br /><br>lean and obese subjects, quantified by confocal microscopy.<br /><br>The third main outcome is the microvascular vasoreactivity of human muscle<br /><br>arterioles as measured in pressure myography ex vivo.<br /><br>The fourth main outcome is the effects of mPAT on the insulin signalling<br /><br>cascade, as measured by Western blotting of mPAT and comparing results of<br /><br>Western blots from subjects with a normal vasoreactivity and those of subjects<br /><br>with perturbed vasoreactivity.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>N/A</p><br>