The role of perivascular adipose tissue in the regulation of insulin sensitivity through muscle microvascular recruitment in non-obese and obese individuals
- Conditions
- Diabetes100192801001265310047066
- Registration Number
- NL-OMON42523
- Lead Sponsor
- Vrije Universiteit Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 30
- Male
- Caucasian
- age 18-60 years
- BMI is *23 and <40 kg/m2
To obtain enough variation between individuals 30 individuals with a wide range of BMI will be recruited:
o 10 individuals with a BMI 23-28
o 10 individuals with a BMI 28-34
o 10 individuals with a BMI 34-40
- Documented CVD
- Diabetes mellitus
- Stage 2 hypertension (resting blood pressure >160/100 mmHg)
- History of severe inflammatory conditions in the past 15 years
- Recent infections
- recent history (<12 months) of high alcohol use > 4 U/day, more than 3 days in a row
- Use of medication potentially affection insulin sensitivity, microvascular function or inflammation
- Use of anticoagulants (types Warfarin and Coumadin derivatives) that can increase the risk of bleeding during the muscle biopsy (exception is made for aspirin).
- Malignancies (except those of the skin), renal and hepatic diseases.
- Smoking
- Recent (<6 months) marked (>10%) changes in body weight.
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Functional and inflammatory properties of PVAT will be quantified and<br /><br>correlations with whole-body and microvascular insulin sensitivity will be<br /><br>analysed. Vasoreactivity of skeletal muscle arterioles with and without<br /><br>perivascular adipose tissue will be studied. We will measure markers of<br /><br>low-grade systemic inflammation and adipocytokines (IL-6, IL-8, leptin, TNF-*,<br /><br>MCP-1, adiponectin, resistin and PAI-1, fibrinogen) .<br /><br>Pearson's and Spearman correlation analyses will be conducted as appropriate to<br /><br>select the variable that is best correlating with insulin sensitivity.<br /><br>step-wise multivariate linear regressions will be performed to examine the<br /><br>relations between two variables, controlling for anthropometry and systemic<br /><br>inflammation, and standardized betas (B) are reported. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Pearson's and Spearman correlation analyses will be conducted as appropriate.<br /><br>Correlations of microvascular recruitment in the heart will be correlated in a<br /><br>regression analysis using PVAT properties, anthropometric properties and<br /><br>inflammatory properties. </p><br>