The Role of Branched-chain Amino Acids (BCAAs) in the Stimulation of Skeletal Muscle Protein Synthesis and Glucose Uptake in Healthy Young Volunteers
Overview
- Phase
- Not Applicable
- Intervention
- Ingestion of an amino acid supplement
- Conditions
- Healthy
- Sponsor
- Wageningen University
- Enrollment
- 24
- Locations
- 1
- Primary Endpoint
- Postprandial muscle protein synthesis
- Status
- Completed
- Last Updated
- 2 months ago
Overview
Brief Summary
The present study aims to quantify the insulin sensitivity and the skeletal muscle protein synthetic response to consuming a test drink containing all amino acids or all amino acids except for BCAAs.
Detailed Description
Twenty-eight healthy, young volunteers will receive a stable isotope tracer infusion (7.5h) combined with repeated blood and muscle sampling, to measure muscle protein synthesis rate in the postabsorptive state following ingestion of a drink containing all amino acids (n=14) or all amino acids except for BCAAs (n=14).
Investigators
Marlou Dirks
Assistant Professor
Wageningen University
Eligibility Criteria
Inclusion Criteria
- •Aged from 18-35 years
- •18.5 \< Body mass index \< 30 kg·m2
- •Recreationally active (performing non-competitive physical exercise at least one time per week for minimally 30 minutes)
Exclusion Criteria
- •Diabetes (Type 1, Type 2, or genetic form of diabetes)
- •Any diagnosed cardiovascular (heart) disease or high blood pressure (≥140 mmHg systolic and/or ≥90 mmHg diastolic)
- •Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices)
- •Known allergy to lidocaine
- •Regular use of dietary protein and/or amino acid supplements (\>3 times per week)
- •Currently involved in a structured progressive resistance training programme (\>3 times per week)
- •A personal or family history of thrombosis (clots), epilepsy, seizures, or schizophrenia.
- •Any previous motor disorders or disorders in muscle and/or lipid metabolism
- •Presence of an ulcer in the stomach or gut and/or strong history of indigestion
- •Known severe kidney problems
Arms & Interventions
NoBCAA
Amino acid drink containing all amino acids except for BCAAs.
Intervention: Ingestion of an amino acid supplement
FullAA
Amino acid drink containing all amino acids
Intervention: Ingestion of an amino acid supplement
Outcomes
Primary Outcomes
Postprandial muscle protein synthesis
Time Frame: 4 hours
Muscle protein synthesis rate (FSR) prior to and following ingestion of an amino acid drink
Secondary Outcomes
- Forearm amino acid kinetics(Prior to and for 4 hours following amino acid intake)
- Forearm muscle glucose uptake(7.5 hours)
- Whole-body insulin sensitivity(Four hours following ingestion of an amino acid drink)