BCAAs, Muscle Protein Synthesis and Glucose Uptake

Not Applicable

Recruiting

• Conditions: Healthy
• Interventions: Dietary Supplement: Ingestion of an amino acid supplement

• Registration Number: NCT06218264
• Lead Sponsor: Wageningen University

Brief Summary

The present study aims to quantify the insulin sensitivity and the skeletal muscle protein synthetic response to consuming a test drink containing all amino acids or all amino acids except for BCAAs.

Detailed Description

Twenty-eight healthy, young volunteers will receive a stable isotope tracer infusion (7.5h) combined with repeated blood and muscle sampling, to measure muscle protein synthesis rate in the postabsorptive state following ingestion of a drink containing all amino acids (n=14) or all amino acids except for BCAAs (n=14).

Study Timeline

• Status Verified: 2024-01
• Study Start: 2024-01-10
• Study First Submitted: 2024-01-11
• Study First Submitted QC: 2024-01-11
• Last Update Submitted: 2024-01-11
• Study First Posted: 2024-01-23
• Last Update Posted: 2024-01-23
• Primary Completion: 2025-12-31
• Study Completion: 2025-12-31

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 28

Inclusion Criteria

• Aged from 18-35 years
• 18.5 < Body mass index < 30 kg·m2
• Recreationally active (performing non-competitive physical exercise at least one time per week for minimally 30 minutes)

Exclusion Criteria

• Smoking
• Diabetes (Type 1, Type 2, or genetic form of diabetes)
• Any diagnosed cardiovascular (heart) disease or high blood pressure (≥140 mmHg systolic and/or ≥90 mmHg diastolic)
• Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices)
• Known allergy to lidocaine
• Regular use of dietary protein and/or amino acid supplements (>3 times per week)
• Currently involved in a structured progressive resistance training programme (>3 times per week)
• A personal or family history of thrombosis (clots), epilepsy, seizures, or schizophrenia.
• Any previous motor disorders or disorders in muscle and/or lipid metabolism
• Presence of an ulcer in the stomach or gut and/or strong history of indigestion
• Known severe kidney problems
• Pregnant or breastfeeding
• Unable to give consent

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
FullAA	Ingestion of an amino acid supplement	Amino acid drink containing all amino acids
NoBCAA	Ingestion of an amino acid supplement	Amino acid drink containing all amino acids except for BCAAs.

Primary Outcome Measures

Name	Time	Method
Postprandial muscle protein synthesis	4 hours	Muscle protein synthesis rate (FSR) prior to and following ingestion of an amino acid drink

Secondary Outcome Measures

Name	Time	Method
Forearm amino acid kinetics	Prior to and for 4 hours following amino acid intake	Plasma phenylalanine kinetics using a stable isotope tracer infusion
Forearm muscle glucose uptake	7.5 hours	Insulin sensitivity, measured as forearm glucose uptake, prior to and following ingestion of an amino acid drink
Whole-body insulin sensitivity	Four hours following ingestion of an amino acid drink	Whole-body insulin sensitivity, calculated using a modified Matsuda index

Trial Locations

Locations (1)

Wageningen University and Research; 🇳🇱 Wageningen, Gelderland, Netherlands