Follicular Fluid Sialic Acid Levels in IVF Cycles

Not Applicable

Completed

• Conditions: in Vitro Fertilization
• Interventions: Diagnostic Test: in vitro fertilization

• Registration Number: NCT03745027
• Lead Sponsor: Kanuni Sultan Suleyman Training and Research Hospital

Brief Summary

This cross-sectional study was conducted at the Reproductive Endocrinology and Invitro Fertilization (REI) unit of our hospital, following the approval of the hospital's ethics committee. Patients who underwent gonadotropin-releasing hormone agonist (GnRHa) treatment were enrolled in the study. During oocyte retrieval, follicular fluids of mature follicles (≥18 mm) were collected and pooled for each patient. The thiobarbituric acid method was used for the sialic acid levels and the absorbance values were measured by a spectrophotometer at 549 nm.

Detailed Description

This cross-sectional study was conducted at the Reproductive Endocrinology and Invitro Fertilization (REI) unit our hospital, following the approval of the hospital's ethics committee. The inclusion criteria were as follows; under 40 years of age, normal thyroid and prolactin hormone levels, a basal follicle stimulating hormone (FSH) level \<10 mIU/mL, BMI of 19-30 kg/m2. Exclusion criteria: a history of ovarian surgery, endometriosis, uterine anomaly, any endocrine disease including congenital adrenal hyperplasia, smokers, use of any medications and hormones in the last 3 months. Before the IVF treatment serum follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), thyroid-stimulating hormone (TSH), and estradiol (E2) levels were measured on the third day of the cycle. Age, body mass index, duration of stimulation, duration of infertility, the total dose of gonadotropins, E2 on the day of hCG administration, number of oocytes retrieved, fertilization rate, and pregnancy rate were recorded.

All patients underwent gonadotropin-releasing hormone agonist (GnRHa) treatment. Oocyte retrieval was done 36 hours after the hCG injection and ICSI was used for all patients. Embryo transfer was performed 48-72 hours after the oocyte retrieval.

During oocyte retrieval, follicular fluids of mature follicles (≥18 mm) were collected from the first follicle entered and were aspirated for each patient. Follicle aspirates, which were not clear and contaminated with blood, were excluded. Total and free sialic acid levels were measured in the follicular fluid.

Study Timeline

• Study Start: 2014-01-01
• Primary Completion: 2016-01-01
• Study Completion: 2016-01-01
• Status Verified: 2018-11
• Study First Submitted: 2018-11-10
• Study First Submitted QC: 2018-11-16
• Study First Posted: 2018-11-19
• Last Update Submitted: 2019-02-24
• Last Update Posted: 2019-02-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 87

Inclusion Criteria

• under 40 years of age,
• normal thyroid and prolactin hormone levels,
• a basal follicle stimulating hormone (FSH) level <10 mIU/mL,
• body mass index (BMI) of 19-30 kg/m2

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Exclusion Criteria

• a history of ovarian surgery, endometriosis, uterine anomaly,
• any endocrine disease including congenital adrenal hyperplasia,
• smokers,
• use of any medications and hormones in the last 3 months.

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
In vitro fertilization	in vitro fertilization	Paients undergoing in vitro fertilization with Gonadotropin-releasing Hormone agonist. Follicular fluid sialic acid levels will be measured.

Primary Outcome Measures

Name	Time	Method
Number of patients who become pregnant	during procedure	Number of patients who become pregnant will be recorded

Trial Locations

Locations (1)

Kanuni SSRTH; 🇹🇷 Istanbul, Turkey