Metabolic Availability of Three Protein Sources in Older and Younger Men as Measured With the Indicator Amino Acid Oxidation Method
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 28
- 试验地点
- 2
- 主要终点
- Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method.
研究概览
简要总结
The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 80 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Gender: Male Age: 20-35 years or 65-80 years Body Mass Index (BMI): 18.5 - 30.0 kg/m2 Healthy as assessed with a questionnaire Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) Willing to comply with study procedures and use of data Having given written informed consent
排除标准
- •Chronic disease, for example:
- •Diabetes mellitus / being treated for high blood glucose
- •Severe cardiovascular disease (exception: hypertension)
- •Hepatic disease (e.g. hepatitis)
- •Renal disease
- •Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding)
- •Pancreatitis
- •History of medical or surgical events that may affect GI function, and the study outcomes or disease risk for participants, for example:
- •Bariatric surgery
- •Gastrointestinal tract surgery
- •Digestive tract disorder
- •Chewing problems
- •History of deep venous thrombosis without anti-coagulation medication
- •Medicine use that interferes with, protein metabolism, GI function and the study outcomes, for example:
- •Glucose lowering drugs
- •Proton pump inhibitors
- •Laxatives
- •Habits that interfere with the study outcomes:
- •Protein supplement use (current use or less than 2 weeks past use)
- •Alcohol consumption >21 units/week and/or >4/day
- •Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet
- •Moderate to high intense physical activity for more than 5 hours a week
- •Self-reported allergy or intolerance to the tested products
- •Weight loss of more than 3 kg in the 3 months prior to study screening
- •Current participation in other research and <2 months prior participation in other research
- •Not having a general practitioner
- •Not willing to accept information-transfer concerning participation in the study , or information regarding his health to his general practitioner
- •Working or doing an MSc- thesis at the department of Human Nutrition and Health at Wageningen University & Research
研究组 & 干预措施
Methionine in black beans in older men
This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine.
干预措施: Black beans (Other)
Methionine in black beans in younger men
This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine.
干预措施: Black beans (Other)
结局指标
主要结局
Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method.
时间窗: 1 measurement to be calculated from repeated measurements on several test days separated by 1 week
Indicator amino acid oxidation method is used to measure metabolic availability of the limiting amino acid by measuring the oxidation of the 13C-labelled indicator amino acid in expired breath as 13CO2 when giving different levels of the limiting amino acid. Methionine metabolic availability will be assessed in black beans and lysine metabolic availability in milk and sorghum.
次要结局
未报告次要终点
研究者
Marco Mensink
Assistant Professor
Wageningen University