Metabolic Availability in Older and Younger Men

Not Applicable

Recruiting

• Conditions: Ageing
• Interventions: Other: Black beans; Other: Milk; Other: Sorghum

• Registration Number: NCT06332651
• Lead Sponsor: Wageningen University

Brief Summary

The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2023-12-19
• Study Start: 2024-02-19
• Status Verified: 2024-03
• Study First Submitted QC: 2024-03-19
• Last Update Submitted: 2024-03-19
• Study First Posted: 2024-03-27
• Last Update Posted: 2024-03-27
• Primary Completion: 2024-12-31
• Study Completion: 2025-04-01

Recruitment & Eligibility

• Status: RECRUITING
• Sex: Male
• Target Recruitment: 28

Inclusion Criteria

Gender: Male Age: 20-35 years or 65-80 years Body Mass Index (BMI): 18.5 - 30.0 kg/m2 Healthy as assessed with a questionnaire Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) Willing to comply with study procedures and use of data Having given written informed consent

Exclusion Criteria

Chronic disease, for example:

• Diabetes mellitus / being treated for high blood glucose
• Severe cardiovascular disease (exception: hypertension)
• Hepatic disease (e.g. hepatitis)
• Renal disease
• Cancer
• Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding)
• Pancreatitis

History of medical or surgical events that may affect GI function, and the study outcomes or disease risk for participants, for example:

• Bariatric surgery
• Gastrointestinal tract surgery
• Digestive tract disorder
• Chewing problems
• History of deep venous thrombosis without anti-coagulation medication

Medicine use that interferes with, protein metabolism, GI function and the study outcomes, for example:

• Glucose lowering drugs
• Proton pump inhibitors
• Laxatives

Habits that interfere with the study outcomes:

• Protein supplement use (current use or less than 2 weeks past use)
• Smoking
• Drug use
• Alcohol consumption >21 units/week and/or >4/day
• Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet
• Moderate to high intense physical activity for more than 5 hours a week

Other:

• Self-reported allergy or intolerance to the tested products
• Weight loss of more than 3 kg in the 3 months prior to study screening
• Current participation in other research and <2 months prior participation in other research
• Not having a general practitioner
• Not willing to accept information-transfer concerning participation in the study , or information regarding his health to his general practitioner
• Working or doing an MSc- thesis at the department of Human Nutrition and Health at Wageningen University & Research

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Methionine in black beans in younger men	Black beans	This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine.
Lysine milk or sorghum in younger men	Milk	This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine.
Methionine in black beans in older men	Black beans	This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine.
Lysine in milk or sorghum in older men	Milk	This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine.
Lysine in milk or sorghum in older men	Sorghum	This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine.
Lysine milk or sorghum in younger men	Sorghum	This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine.

Primary Outcome Measures

Name	Time	Method
Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method.	1 measurement to be calculated from repeated measurements on several test days separated by 1 week	Indicator amino acid oxidation method is used to measure metabolic availability of the limiting amino acid by measuring the oxidation of the 13C-labelled indicator amino acid in expired breath as 13CO2 when giving different levels of the limiting amino acid. Methionine metabolic availability will be assessed in black beans and lysine metabolic availability in milk and sorghum.

Trial Locations

Locations (1)

Wageningen University & Research; 🇳🇱 Wageningen, Gelderland, Netherlands