Metabolic availability of three protein sources in older and younger men as measured with the indicator amino acid oxidation method

Recruiting

• Conditions: veroudering; ageing; senescence

• Registration Number: NL-OMON56568
• Lead Sponsor: Wageningen Universiteit

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Last Update Posted: 9 April 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 28

Inclusion Criteria

- Gender: male
- Age: 20-35 years or 65-80 years
- Body Mass Index (BMI): 18.5 - 30.0 kg/m2
- Healthy as assessed with a questionnaire
- Regular and normal eating habits as assessed with a Food Frequency
Questionnaire (FFQ)
- Willing to comply with study procedures and use of data
- Having given written informed consent

Exclusion Criteria

Chronic disease, for example: - Diabetes mellitus / being treated for high
blood glucose - Severe cardiovascular disease (exception: hypertension) -
Hepatic disease (e.g. hepatitis) - Renal disease - Cancer - Bowel disease (e.g.
inflammatory bowel disease, ulcers, bleeding) - Pancreatitis
History of medical or surgical events that may affect GI function, study
outcomes or disease risk for participants, for example: - Bariatric surgery -
Gastrointestinal tract surgery - Digestive tract disorder - Chewing problems -
History of deep venous thrombosis without anti-coagulation medication
Medicine use that interferes with, protein metabolism, GI function and the
study outcomes, for example: - Glucose lowering drugs - Proton pump inhibitors
- Laxatives
Habits that interfere with the study outcomes: - Protein supplement use
(current use or less than 2 weeks past use) - Smoking - Drug abuse - Alcohol
consumption >21 units/week or >4/day - Following a weight-loss diet, medically
prescribed diet or other diet with a low calorie intake or an unbalanced
nutrient intake like a vegan or very low carbohydrate diet - Moderate to high
intense physical activity for more than 5 hours a week
Other: - Self-reported allergy or intolerance to the tested products - Weight
loss of more than 3 kg in the 3 months prior to study screening - Current
participation in other research and <2 months prior participation in other
research - Not having a general practitioner - Not willing to accept
information-transfer concerning participation in the study, or information
regarding his or her health to his general practitioner - Working or doing a
MSc thesis at the department of Human Nutrition and Health at Wageningen
University and Research

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>13C-enrichment in breath samples will be expressed in APE. CO2 production rate<br /><br>(VCO2) will be measured by indirect calorimetry and expressed in mL/min. The<br /><br>rate of 13CO2 production (F13CO2) will be calculated using these two<br /><br>measurements. The slope for the graded intakes of the limiting amino acid and<br /><br>the oxidation level will be constructed for the reference and the test<br /><br>proteins. The ratio between the reference and test protein will be calculated<br /><br>to represent metabolic availability of the limiting amino acid in each protein<br /><br>source. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Not applicable</p><br>