Difference in digestibility of three protein sources between older and younger adults as measured with the dual tracer method
- Conditions
- verouderingageingsenescence
- Registration Number
- NL-OMON51395
- Lead Sponsor
- Wageningen Universiteit
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 20
- Age: 20-35 years or 65-80 years
- Body Mass Index (BMI): 20.0 - 30.0 kg/m2
- Veins suitable for blood sampling
- Healthy as assessed with a questionnaire
- Regular and normal Dutch eating habits as assessed with a questionnaire
- Having given written informed consent
- Willing to comply with study procedures
- Accept use of all encoded data, including publication, and the confidential
use and storage of all data for 15 years.
- Chronic disease, for example:
o Diabetes mellitus, being treated for high blood glucose or increased fasting
blood glucose (> 6.7 mmol/l in finger prick blood)
o Active cardiovascular disease
o Hepatic disease (e.g. hepatitis)
o Renal disease
o Cancer
o Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding)
o Pancreatitis
- History of medical or surgical events that may affect GI function and the
study outcomes, for example:
o Bariatric surgery
o Gastrointestinal tract surgery
o Digestive tract disorder
o Chewing problems
- Medicine use that interferes with GI function and the study outcomes, for
example:
o Glucose lowering drugs
o Proton pump inhibitors
o Laxatives
- Habits that interfere with the study outcomes:
o Probiotics and/or protein supplement use
o Smoking
o Drug abuse
o Alcohol consumption for men >21 units/week and >4/day and for women >14
units/week and >3/day
o Following a weight-loss diet, medically prescribed diet or other diet with a
low calorie intake or an unbalanced nutrient intake like a vegan or very low
carbohydrate diet
o Moderate to high intense physical activity for more than 5 hours a week
o Difficulties with eating breakfast in the morning
- Other:
o Self-reported allergy or intolerance to the tested products (milk, sorghum,
black beans)
o Weight loss of more than 3 kg in the month prior to study screening
o Being pregnant or breastfeeding
o For men: Hb <8,5 mmol/l and for women: Hb <7,5 mmol/l
o Recent blood donation (<2 months prior to start of the study) and planning to
donate blood (<4 months after the termination of the study)
o Current participation in other research and <2 months previous participation
in other research
o Not having a general practitioner
o Not willing to accept information-transfer concerning participation in the
study, or information regarding his or her health, like laboratory results and
eventual adverse events to and from his general practitioner
o Working at the department of Human Nutrition and Health at Wageningen
University & Research
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Blood samples will be drawn every hour until the 5th hour and every 30 minutes<br /><br>afterwards up to 8 hours. Plasma and meal amino acids enrichment will be<br /><br>measured with gas chromatography - isotope ratio mass spectrometry (GC-IRMS)<br /><br>for both isotopes. The ratio between 2H and 13C labelled amino acids in both<br /><br>meal and blood plasma will be determined and amino acid digestibility of the<br /><br>tested protein source will be calculated. </p><br>
- Secondary Outcome Measures
Name Time Method <p>- To determine the difference in amino acid digestibility between milk, sorghum<br /><br>and black beans within age groups.<br /><br>- To explore the difference in blood amino acids kinetics between milk, sorghum<br /><br>and black beans and between older and younger adults.<br /><br>- To investigate the association between gut health as measured with blood<br /><br>markers and amino acid digestibility of milk, sorghum and black beans. </p><br>