Exercise in Hypoxia and Nitrate Supplementation in Athletes: is the Whole Greater Than the Sum of Its Parts?
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Hypoxia
- Sponsor
- University of Trás-os-Montes and Alto Douro
- Enrollment
- 30
- Locations
- 1
- Primary Endpoint
- Performance adaptations
- Status
- Completed
- Last Updated
- last year
Overview
Brief Summary
Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions.
Detailed Description
Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions. It is hypothesized that the combined effect of these interventions will promote an additional performance and health improvement in this population at sea-level and altitude conditions, compared with the single effect.
Investigators
Ana Sousa
PhD
Advanced Bionics
Eligibility Criteria
Inclusion Criteria
- •trained male subjects, regularly involved in competitive running, cycling and/ or triathlon events (4-5 times per week) in the past five years
Exclusion Criteria
- •smoking or other chronic issues
- •using medication or dietary supplements in the last 3 months, or during the duration of the study
- •being exposed to altitude (≥2000 m) in the last 3 months
Outcomes
Primary Outcomes
Performance adaptations
Time Frame: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
VO2 kinetics (time constant and amplitude of fast and slow oxygen components)
Cardiovascular and neuromuscular adaptations
Time Frame: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Muscular oxygen saturation (SmO2)
Skeletal muscle adaptations
Time Frame: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Mitochondrial and capillary density, capillary-to-fibre ratio and fibre cross-section area - cryosections cut with a cryostat and further samples analysed by fluorescence microscopy
Haematological adaptations
Time Frame: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Plasma \[NO2-\] and \[NO3-\], erythropoietin (EPO), haemoglobin (Hb) and haematocrit (Hct) - all assays on plasma sample analysed by spectrophometry
Oxidative stress and antioxidant markers adaptations
Time Frame: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Nitrotyrosine, malondialdehyde, ferric-reducing antioxidant power and superoxide dismutase concentrations - all assays on plasma sample analysed by spectrophometry
Secondary Outcomes
- Sleep and cardiac autonomic activity adaptations(Moment #1 (2 weeks pre-intervention - baseline) and moment #2 (4 weeks intervention))