Exercise in Hypoxia and Nitrate Supplementation in Athletes: is the Whole Greater Than the Sum of Its Parts?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Performance adaptations
研究概览
简要总结
Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions.
详细描述
Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions. It is hypothesized that the combined effect of these interventions will promote an additional performance and health improvement in this population at sea-level and altitude conditions, compared with the single effect.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •trained male subjects, regularly involved in competitive running, cycling and/ or triathlon events (4-5 times per week) in the past five years
排除标准
- •smoking or other chronic issues
- •using medication or dietary supplements in the last 3 months, or during the duration of the study
- •being exposed to altitude (≥2000 m) in the last 3 months
研究组 & 干预措施
HNO: High intensity interval training (HIIT) + nitrate supllementation (NO3-).
Performed 12 high-intensity interval-training (HIIT) sessions during a 4-week period (3 sessions/ week) under normobaric hypoxia conditions (FiO2=~13%, ~3000 m) with NO3- supplement.
干预措施: Nitrate + Hypoxia (Combination Product)
HPL: High intensity interval training (HIIT) + placebo supllementation.
Performed 12 high-intensity interval-training (HIIT) sessions during a 4-week period (3 sessions/ week) under normobaric hypoxia conditions (FiO2=~13%, ~3000 m) with placebo supplement.
干预措施: Placebo + Hypoxia (Dietary Supplement)
CON: High intensity interval training (HIIT) + placebo supllementation.
Performed 12 high-intensity interval-training (HIIT) sessions during a 4-week period (3 sessions/ week) under normoxic conditions (FiO2=~20.9%) with placebo supplement.
干预措施: Control (Other)
结局指标
主要结局
Performance adaptations
时间窗: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Time-to-task failure (time sustained)
Cardiovascular and neuromuscular adaptations
时间窗: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Maximal oxygen uptake (VO2max: absolute and relative value)
Skeletal muscle adaptations
时间窗: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Oxidative enzyme activity (cytochrome oxidase subunit IV), citrate synthase, transcription factors (HIF-1alpha and PGC-1alpha) and GLUT4 - assessed through standard enzymatic fluorometric assay and western bllotting
Haematological adaptations
时间窗: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Blood pressure (Hmmg), mean arterial pressure (calculated as 1/3 \* systolic pressure + 2/3 \* diastolic pressure)
Oxidative stress and antioxidant markers adaptations
时间窗: Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)
Nitrotyrosine, malondialdehyde, ferric-reducing antioxidant power and superoxide dismutase concentrations - all assays on plasma sample analysed by spectrophometry
次要结局
- Sleep and cardiac autonomic activity adaptations(Moment #1 (2 weeks pre-intervention - baseline) and moment #2 (4 weeks intervention))
研究者
Ana Sousa
PhD
Advanced Bionics