Elevated Plasma Levels of TIMP-1 in Patients With Rotator Cuff Tear

Completed

• Conditions: Rotator Cuff Tear
• Interventions: Procedure: Blood sampling

• Registration Number: NCT01562145
• Lead Sponsor: University Hospital, Linkoeping

Brief Summary

The aim of the study was to investigate the levels of matrixmetalloproteinases and their inhibitors in blood samples from patients with rotator cuff tears and compare with blood samples from healthy matched controls.

Detailed Description

Background: Extracellular matrix remodeling is altered in rotator cuff tears, partly due to altered expression of matrix metalloproteinases (MMPs) and their inhibitors. It is unclear if this altered expression can be traced as changes in plasma protein levels. We measured the plasma level of MMPs and their tissue inhibitors (TIMPs) in patients with rotator cuff tears.

Material and methods: Blood samples were collected from 17 patients, median 61 (range 39-77) years, with sonographically verified rotator cuff tears (partial- or full-thickness). These were compared with 16 gender and age matched control persons with sonographically intact rotator cuffs. Plasma levels of MMPs and TIMPs were measured simultaneously using Luminex technology.

Study Timeline

• Study Start: 2009-01
• Primary Completion: 2010-02
• Study Completion: 2010-02
• Status Verified: 2012-03
• Study First Submitted: 2012-03-21
• Study First Submitted QC: 2012-03-21
• Study First Posted: 2012-03-23
• Last Update Submitted: 2012-03-23
• Last Update Posted: 2012-03-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 34

Inclusion Criteria

• degenerative rotator cuff tear verified by ultrasound

Exclusion Criteria

• systemic joint disease such as rheumatoid arthritis
• a fracture non-union
• dupuytrens disease
• frozen shoulder
• tendinosis or rupture of any other tendons than in the rotator cuff
• disorders of the spine such as disc disease,idiopathic scoliosis, spondylitis
• cerebral or cardiovascular disease during the past year
• abdominal or bowel disease
• surgery or trauma during the past year
• any infection during the last month
• malignancy
• treatment for the last month with medications that may affect MMPs or TIMPs (tetracycline, bisphosphonates, anti-inflammatory drugs, statins)
• vigorous physical activity during the last 24 hours
• inability to understand written and spoken Swedish

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Patients with rotator cuff tears	Blood sampling	Patients with ultrasound verified rotator cuff tears
Healthy controls	Blood sampling	Age and gender matched controls with ultrasound verified intact rotator cuff

Primary Outcome Measures

Name	Time	Method
Level of MMP and TIMP in plasma	Measaured at one time point	Four mL venous blood samples were collected from all study participants after inclusion, and centrifuged to plasma that was stored at -70 degrees until the analysis.

Trial Locations

Locations (1)

University clinic of orthopeadics Linkoeping; 🇸🇪 Linkoeping, Oestergoetland, Sweden