Antibiotic Resistance in Oral Biofilms: Does the oral biofilm constitute a reservoir for antibiotic resistant bacteria and antibiotic resistance genes?

• Conditions: antibiotic resistant bacteria; antibiotic resistance genes; caries; periodontitis; K02; K05.3; Dental caries; Chronic periodontitis

• Registration Number: DRKS00013119
• Lead Sponsor: Klinik für Zahnerhaltungskunde und ParodontologieUniversitätsklinikum Freiburg

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2018-11-09
• Study Completion: 2020-12-31
• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Complete
• Sex: All
• Target Recruitment: 180

Inclusion Criteria

At each research center 20 healthy volunteers, 20 patients with caries and 20 patients with periodontitis are being included.

- Other than that subjects are generally healthy.

- No antibiotic treatment within the last six months.

Definition healthy volunteers:
Subjects exhibit periodontal sound conditions and 28 caries- and restoration-free teeth (DMFT = 0)

Definition patients with active caries:
Subjects exhibit at least two active dentine caries lesions and have undergone at least two invasive restorative dental treatments in the last two years.

Definition patients with periodontitis:
Subjects are being diagnosed with a generalized moderate localized severe chronic Periodontitis or generalized severe chronic Periodontitis (classification according to Armitage, 1999)
No periodontal therapy and no supporting periodontal therapy (SPT) within the last 24 months.

Exclusion Criteria

- Antibiotic treatment during the study
- Pregnancy
- Diabetes
- Pathologies of the oral mucosa
- Severe medical conditions (i.e. HIV, immunosuppression)
- Pharmacological treatment affecting the gingival conditions (anticonvulsants, antihypertensive agents)
- Limited inclusion of smokers (20% per group)

Study & Design

• Study Type: observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
1. Description of the microbiome in the subgroups<br>2. Description of the resistome in the subgroups<br>3. Determination of phenotypic resistance by culture technique and antibiotic susceptibility testing with agar diffusion methods