Postprandial Plasma Amino Acid Concentrations After Dairy Consumption

Not Applicable

Completed

• Conditions: Protein Digestion Kinetics
• Interventions: Other: skimmed milk (pasteurized); Other: skimmed yoghurt; Other: whey protein; Other: non-homogenized full-fat milk (pasteurized); Other: full-fat milk (UHT); Other: skimmed milk (UHT); Other: full-fat cheese (semi-matured); Other: micellar casein

• Registration Number: NCT02546141
• Lead Sponsor: NIZO Food Research

Brief Summary

This study was designed to obtain information about the change in postprandial amino acids in blood over time, after consumption of different dairy products, in an elderly population and how this relates to gastric emptying rate and feelings of satiety.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2015-09-08
• Study First Submitted QC: 2015-09-08
• Study First Posted: 2015-09-10
• Study Start: 2015-10
• Primary Completion: 2015-12
• Study Completion: 2015-12
• Status Verified: 2016-01
• Last Update Submitted: 2016-01-11
• Last Update Posted: 2016-01-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

• Age over 60y
• BMI 21-30 kg/m2
• Non-smoking
• Healthy as assessed by the NIZO lifestyle and health questionnaire
• Regular and normal Dutch eating habits as assessed by the NIZO lifestyle and health questionnaire (3 main meals per day)
• Veins suitable for cannulation (blood sampling)

Exclusion Criteria

• Participation in any clinical trial including blood sampling and/or administration of substances up to 30 days before Day 01 of this study
• Having a history of medical or surgical events that may significantly affect the study outcome, including: Inflammatory bowel disease, hepatitis, pancreatitis, ulcers, gastrointestinal or rectal bleeding; major gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel resection; known or suspected gastrointestinal disorders, colon or GI tract cancer
• Use of the following medication: glucose lowering drugs, insulin; medication that may impact gastric emptying (e.g. gastric acid inhibitors or laxatives)
• Diagnosed with diabetes, being treated for high blood glucose or increased fasting blood glucose (> 7 mmol/l in finger prick blood) as assessed during screening visit
• For men: Hb <8,5 mmol/l as assessed during screening visit; for women: Hb <7,5 mmol/l.
• Use of protein supplements
• Mental status that is incompatible with the proper conduct of the study
• A self-reported reported food allergy or sensitivity to dairy ingredients
• A self-reported allergy or sensitivity to acetaminophen
• Alcohol consumption > 28 units/week and 4/day
• Reported unexplained weight loss or weight gain of > 3 kg in the month prior to pre-study screening, or intention to lose weight during the study period
• Reported slimming or medically prescribed diet
• Recent blood donation (<1 month prior to Day 01 of the study)
• Not willing or afraid to give up blood donation during the study
• Personnel of NIZO food research or Wageningen University, department of Human Nutrition, their partner and their first and second degree relatives
• Not having a general practitioner
• Not willing to accept information-transfer concerning participation in the study, or information regarding his health, like laboratory results and eventual adverse events to and from his general practitioner

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
skimmed milk (pasteurized)	skimmed milk (pasteurized)	portion size that contains 25 g of protein, oral, single administration
skimmed yoghurt	skimmed yoghurt	portion size that contains 25 g of protein, oral, single administration
whey protein	whey protein	portion size that contains 25 g of protein, oral, single administration
non-homogenized full-fat milk (pasteurized)	non-homogenized full-fat milk (pasteurized)	portion size that contains 25 g of protein, oral, single administration
full-fat milk (UHT)	full-fat milk (UHT)	portion size that contains 25 g of protein, oral, single administration
skimmed milk (UHT)	skimmed milk (UHT)	portion size that contains 25 g of protein, oral, single administration
full-fat cheese (semi-matured)	full-fat cheese (semi-matured)	portion size that contains 25 g of protein, oral, single administration
micellar casein	micellar casein	portion size that contains 25 g of protein, oral, single administration

Primary Outcome Measures

Name	Time	Method
Cmax for postprandial individual plasma amino acids	0-300 min after consumption
iAUC for postprandial individual plasma amino acids	0-300 min after consumption
Tmax for postprandial individual plasma amino acids	0-300 min after consumption

Trial Locations

Locations (1)

NIZO food research; 🇳🇱 Ede, Netherlands