The Effect of Sitting and Moderate Exercise on Plasma Triglyceride Elevation After a Meal

Not Applicable

Completed

• Conditions: Atherosclerosis; Metabolic Syndrome
• Interventions: Behavioral: Prolonged sitting without exercise; Behavioral: Prolonged sitting with exercise

• Registration Number: NCT03098446
• Lead Sponsor: University of Texas at Austin

Brief Summary

The purpose of this study is to investigate the effect of 4 days of sitting and moderate exercise on plasma triglyceride elevation after a meal.

Detailed Description

Subjects will undergo three weeks (two intervention, one washout) of testing during which they will participate in two conditions: 1) prolonged sitting (\~14-hours/day) with a 1-hour bout of acute exercise at 65% of pre-intervention VO2max and 2) prolonged sitting (\~14-hours/day) without an acute bout of exercise. During each intervention week, subjects will have 2 days of dietary and activity control, followed by 4 days during which prolonged sitting will occur. On the evening of the 4th day, subjects in the exercise group will perform the acute bout of exercise. On day 5, all subjects will undergo a lipid tolerance test to determine the body's ability to clear triglycerides from the blood as well as insulin/glucose response and substrate oxidation. Blood samples will be assayed for the previously mentioned substances and postprandial gas collection will be analyzed via indirect calorimetry.

Study Timeline

• Study First Submitted: 2017-03-27
• Study First Submitted QC: 2017-03-27
• Study First Posted: 2017-03-31
• Study Start: 2017-04-08
• Primary Completion: 2017-08-11
• Study Completion: 2017-08-11
• Status Verified: 2018-10
• Last Update Submitted: 2018-10-03
• Last Update Posted: 2018-10-05

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

Exclusion Criteria

• cardiovascular problems (e.g. pre-existing heart issues, coronary artery disease, hypertension, etc.)
• respiratory problems
• musculoskeletal problems that prevent prolonged sitting or exercise
• obesity
• susceptibility to fainting

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Prolonged sitting without exercise	Prolonged sitting without exercise	Subjects will be asked to undergo prolonged sitting (\~14-hours/day) for 4 days. Subjects will not be asked to complete the acute bout of exercise to serve as a control.
Prolonged sitting with exercise	Prolonged sitting with exercise	Subjects will be asked to undergo prolonged sitting (\~14-hours/day) for 4 days. On the evening of day 4, they will be asked to run at 65% of VO2max for 1-hour.

Primary Outcome Measures

Name	Time	Method
Plasma Triglycerides	6-hours	Areas under the curve for triglyceride concentration will be calculated for hourly samples from a 6-hour lipid tolerance test

Secondary Outcome Measures

Name	Time	Method
Plasma Insulin	6-hours	Areas under the curve for insulin concentration will be calculated for hourly samples from a 6-hour lipid tolerance test
Plasma Glucose	6-hours	Areas under the curve for glucose concentration will be calculated for hourly samples from a 6-hour lipid tolerance test

Trial Locations

Locations (1)

University of Texas at Austin Human Performance Laboratory; 🇺🇸 Austin, Texas, United States