Distribution of Nutrient Derived Amino Acids

Not Applicable

Completed

• Conditions: Muscle Mass; Protein Synthesis; Protein Intake; Sarcopenia; Diet Modification; Protein Breakdown
• Interventions: Other: Distribution pattern of dietary proteins (minced meat)

• Registration Number: NCT03870425
• Lead Sponsor: Bispebjerg Hospital

Brief Summary

The project examines over several days how the distribution pattern of dietary protein stimulate and affect the protein turnover of important proteins in the aging perspective, such as skeletal muscle proteins.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2019-01-18
• Study First Submitted QC: 2019-03-11
• Study First Posted: 2019-03-12
• Study Start: 2019-04-09
• Primary Completion: 2020-06-30
• Study Completion: 2022-02-28
• Status Verified: 2022-11
• Last Update Submitted: 2022-11-18
• Last Update Posted: 2022-11-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Men and women
• Between 65-80 years of age

Exclusion Criteria

• BMI above 30
• Smokers
• Vegetarians
• Diabetes or other metabolic diseases
• Medical treatment which affects protein turnover
• Gastro-intestinal diseases, reduced kidney or liver function, or chronic inflammation diseases
• Elevated blood-pressure or signs of atherosclerosis
• More than 5 hours of regular exercise each week

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
MINCED-EVEN	Distribution pattern of dietary proteins (minced meat)	Minced meat administered with an even protein distribution pattern
MINCED-SKEWED	Distribution pattern of dietary proteins (minced meat)	Minced meat administered with a skewed protein distribution pattern

Primary Outcome Measures

Name	Time	Method
Muscle protein synthesis	3 days	Myofibrillar FSR measured by deuterated water technique, by which deuterium labeled alanine incorporation into myofibrillar muscle protein synthesis can be measured.
Net protein balance	10 hours	Whole body net protein balance measured during 10 hours acute study. Measured by the application of stable amino acid labeled phenylalanine and tyrosine tracers, by which the protein synthesis, phenylalanine oxidation and protein breakdown can be calculated.

Secondary Outcome Measures

Name	Time	Method
Phenylalanine oxidation	10 hours	Phenylalanine oxidation measured during acute study
Protein synthesis	10 hours	Whole body protein synthesis (%/hour) measured during acute study, by the use of stabel amino acid tracer technique.
Signaling pathways in skeletal muscle	10 hours	Intracellular signaling pathways related to protein turnover. Measured by Western blotting technique from the muscle tissue biopsies taken at 2 hours and 12 hours into the acute study.
D5-phenylalanine incorporation	3 days	D5-phenylalanine incorporation into myofibrillar proteins. Dietary meat proteins are intrinsically labeled with D5-phenylalanine
Protein breakdown	10 hours	Whole body protein breakdown (%/hour) measured during acute study, by the use of stabel amino acid tracer technique.
D5-phenylalanine appearance	12 hours	Plasma D5-phenylalanine enrichment after intake of D5-phenylalanine intrinsically labelled meat proteins
Plasma insulin	12 hours	Plasma insulin concentration during acute study
Muscle protein synthesis	10 hours	Myofibrillar protein synthesis (%/hour) measured during 10 hours of the acute study. Calculated from the difference in D8-phenylalanine incorporation from the muscle biopsies taken at 2 hours and 12 hours into the stable amino acid tracer infusion.

Trial Locations

Locations (1)

Institute of Sports Medicine Copenhagen; 🇩🇰 Copenhagen, Denmark