Eccentric Cycling Exercise With Assistive Devices on Mitochondrial Metabolism in T Lymphocyte

Not Applicable

Completed

• Conditions: There Are no Conditions Under Study. Healthy Volunteers

• Registration Number: NCT06905964
• Lead Sponsor: Chang Gung Memorial Hospital

Brief Summary

Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness.

Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions.

Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.

Detailed Description

Not available

Study Timeline

• Study Start: 2023-02-23
• Primary Completion: 2024-02-23
• Study Completion: 2024-12-31
• Status Verified: 2025-01
• Study First Submitted: 2025-01-08
• Study First Submitted QC: 2025-03-31
• Last Update Submitted: 2025-03-31
• Study First Posted: 2025-04-02
• Last Update Posted: 2025-04-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 30

Inclusion Criteria

• Sedentary lifestyle (exercise<20min/session; <1session/week for a year or longer).
• Nonsmokers, nonusers of medications/vitamins.
• No any cardiopulmonary, neuromuscular, and hematological risks.

Exclusion Criteria

• Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
• Have retabolic or musculoskeletal disease.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Mitochondrial bioenergetics of oxygen consumption rate in T lymphocyte	At baseline and 2 days after completing the 5-week intervention	The bioenergetic health index (BHI) of mitochindria in T lymphocyte was measured. BHI is a parameter for detecting the health status of mitochondrial and efficiency of energy metabolism.
Systemic aerobic fitness (VO2)	At baseline and 2 days after completing the 5-week intervention	VO2max was determined during the exercise testing. VO2max is the maximum of oxygen deliver and utilization.

Trial Locations

Locations (1)

Chang Gung University; 🇨🇳 Guishan, Taoyuan, Taiwan

Chang Gung University

🇨🇳Guishan, Taoyuan, Taiwan