Effect of Exercise on Appetite, Energy Intake, Butyrylcholinesterase Activity and Gut Peptides in Men With Variants of the Obesity-linked FTO rs9939609 Polymorphism
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Loughborough University
- Enrollment
- 24
- Locations
- 1
- Primary Endpoint
- Plasma acylated ghrelin concentrations (N=24)
Study Overview
Brief Summary
Using a database of individuals with FTO genetic data, the study aims to assess the appetite, energy intake, butyrylcholinesterase, gut hormone responses to a bout of moderate- to high intensity exercise in individuals with genetic variations in the FTO gene.
Detailed Description
The fat mass and obesity-associated gene (FTO) rs9939609 A allele is related to obesity, greater food intake and impaired postprandial reduction of ghrelin. Exercise acutely suppresses levels of ghrelin and appetite, yet whether the response differs in people with or without the rs9939609 A allele is unknown. This study assessed the effect of exercise on appetite, appetite-regulatory hormones and energy intake in variants of the FTO rs9939609 polymorphism.
Cohort
The investigators initially recruited 202 subjects to a database and measured FTO rs9939609 genotype. From these subjects, 12 individuals homozygous for the 'obesity-risk' rs9939609 A allele and 12 homozygous for the T allele were recruited.
A sample size of 24 was chosen based on previous data which suggested that a 34 pg/ml reduction in circulating acylated ghrelin during exercise could be detected with > 80% power with a two-tailed t-test whilst assuming a standard deviation of differences of 54 pg/ml.
Familiarisation trial
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •non-smoker, not currently dieting, weight stable for >3 months (self-reported), no personal history of cardiovascular disease, metabolic disease or dyslipidaemia, European ancestry, no psychiatric or medical condition
Exclusion Criteria
- •food allergies, dislike or intolerance of study foods and drinks, irregular eating patterns, use of medication that could influence hormone concentrations
Arms & Interventions
Control
Experimental day where participants rest.
Intervention: Control (Other)
Exercise
Experimental day where participants complete a run.
Intervention: Exercise (Other)
Outcomes
Primary Outcomes
Plasma acylated ghrelin concentrations (N=24)
Time Frame: 24 hours
Measured using ELISA from venous blood samples
Ad-libitum energy intake (N=24)
Time Frame: 24 hours
Measured at laboratory-based meals
Subjective appetite (N=24)
Time Frame: 24 hours
Measured using visual analogue scales
Plasma desacyl ghrelin concentrations (N=24)
Time Frame: 24 hours
Measured using ELISA from venous blood samples
Plasma butyrylcholinesterase activity (N=24)
Time Frame: First hour of main trial (three samples).
Measured using Ellman's reagent protocol
Secondary Outcomes
- Plasma total glucagon-like peptide 1 concentrations (N=24)(24 hours)
- Plasma leptin concentrations (N=24)(Baseline (fasting) sample)
- Plasma total peptide yy concentrations (N=24)(24 hours)
Investigators
Professor David Stensel
Professor of Exercise Metabolism
Loughborough University