Effect of Oral Lactate Ingestion on Appetite Regulation
- Conditions
- Appetitive Behavior
- Interventions
- Other: Sodium LactateOther: Sodium Chloride
- Registration Number
- NCT06379815
- Lead Sponsor
- Wilfrid Laurier University
- Brief Summary
The goal if this study is to determine how oral lactate ingestion affects markers of appetite regulation. Researchers will compare oral lactate ingestion to a sodium chloride placebo. Participants will consume the lactate or placebo solution and then have blood samples and assessments of appetite over the course of 90 min. Free-living energy intake will be measured for 3 days surrounding each experimental session.
- Detailed Description
The purpose of this study is to explore the effects of oral lactate ingestion on appetite regulation in humans. To do this males and females will report to the laboratory for 2 experimental sessions completed in a counterbalanced, double-blinded manner. During one session a lactate solution will be ingested, in another session an equimolar sodium chloride solution will be ingested that matches the osmolarity of the lactate condition. Venous blood samples and subjective appetite perceptions will be obtained at five time points during each experimental session. Energy expenditure will be measured through accelerometers placed on the participants anterior thigh by an investigator on the morning the day before the session and worn over the experimental period (day before, day of, day after) recording their physical activity. Energy intake will be tracked using a smartphone mobile application called Keenoa over the same time period the accelerometers are worn.
Recruitment & Eligibility
- Status
- NOT_YET_RECRUITING
- Sex
- All
- Target Recruitment
- 12
- Deemed healthy using Canadian Society for Exercise Physiology Get Active Questionnaire
- Female participants must be eumenorrheic (menstrual cycle length between 21-35 days)
- Failure to meet inclusion criteria
- Taking supplements or medications known to affect metabolism (e.g., creatine, beta-blockers)
- Currently pregnant or a pregnancy of >3 months within the last 3 years,
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Sodium Lactate Sodium Lactate Participants will received 0.45 g/kg of sodium lactate (SO179, Spectrum Chemical MFG Group) mixed with water. This will be mixed at a ratio of 30 mL per g of lactate. Lemon juice is added to the mixture at a specific ratio to balance the pH of the solution. Crystal light will also be added to avoid any taste differences between beverages. Sodium Chloride Sodium Chloride Participants will receive a equimolar amount of sodium chloride (S9888, MilliporeSigma) mixed with water. The amount of water, lemon juice, and crystal light will be matched to that of the sodium lactate condition.
- Primary Outcome Measures
Name Time Method Acylated ghrelin Pre-ingestion, 30 minutes following ingestion, 45 minutes following ingestion, 60 minutes following ingestion, and 90minutes following ingestion. Acylated ghrelin measured in blood via ELISA.
Total ghrelin Pre-ingestion, 30 minutes following ingestion, 45 minutes following ingestion, 60 minutes following ingestion, and 90minutes following ingestion. Total ghrelin measured in blood via ELISA.
Blood Lactate Pre-ingestion, 30 minutes following ingestion, 45 minutes following ingestion, 60 minutes following ingestion, and 90minutes following ingestion. Lactate measured in blood.
- Secondary Outcome Measures
Name Time Method Free-living energy intake Day 1 (day before the experimental session), Day 2 (day of the experimental session), and Day 3 (day after the experimental session). Free-living energy intake measured using a mobile application Keenoa
Subjective appetite perception Pre-ingestion, 30 minutes following ingestion, 45 minutes following ingestion, 60 minutes following ingestion, and 90minutes following ingestion. Measured using visual analogue scale