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Efficacy of a Whitening Dentifrice on Tooth Discoloration

Phase 4
Completed
Conditions
Tooth Discoloration
Interventions
Other: Whitening toothpaste
Other: Conventional toothpaste
Other: 10% carbamide peroxide
Registration Number
NCT02741427
Lead Sponsor
Federal University of Paraíba
Brief Summary

The purpose of this study was to compare the whitening effect of a toothpaste containing blue pigment with a conventional toothpaste and with at-home tooth bleaching; evaluate the subject's perception about tooth color improvement and; and evaluate the safety and acceptability of these products.

Detailed Description

The study was approved by local Ethical Committee under protocol number CAAE: 035541412.7.0000.5188, and each volunteer signed an informed consent form prior to enrollment in the study.

The sample size calculation was carried out based on a previous study. To detect the bleaching effect with a power of 80% and a one-tailed alpha error of 5%, a sample size of 20 subjects in each treatment group was necessary to detect a 20% difference between groups in shade change. A 20% addiction in subjects' number, taking into consideration potential loss or refusal, gave a total sample size of 75 subjects (25 in each group). The individuals were invited to participate in this clinical trial through advertisement exposed at the Federal University of Paraíba, Brazil.

Basing on eligibility criteria, 75 volunteers were selected to the trial and randomized to one of the three groups of treatment: G1- brushing teeth with a conventional toothpaste; G2- brushing teeth with a silica whitening dentifrice containing blue pigment; and G3 - at-home tooth bleaching with 10% carbamide peroxide. The treatments will be detailed in another section. It was used a simple randomization to allocate the subjects, and a randomization table was prepared in the Excel Program by a member, not directly involved with the clinical part of the study.

To mask the products used in the treatment groups, each toothpaste type was enveloped with an adhesive tape of a different color and the seal of the bleaching gel syringe was removed. The same examiner responsible for subject allocation did this procedure. Thus, testing with the toothpastes was double-blinded, where both evaluator and subject did not know which toothpaste was being used.

The outcomes evaluated were: tooth shade, subjects' perception about tooth color appearance, and safety and acceptability of products tested, which were measured at baseline, 2-weeks, 3-weeks and 4-weeks of the beginning of treatments.

For statistical analysis, data were checked for normal distribution using the Kolmogorov Smirnov test, and then it was applied the appropriated parametric or non-parametric tests. Differences were considered statistically significant when p \< 0.05.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
75
Inclusion Criteria
  • To have six maxillary anterior teeth with a mean shade of C1 or darker (Vitapan Classical, Vita Zahnfabrik, Bad Sackingen, Germany),
Exclusion Criteria
  • With active caries or periodontal disease,
  • Presenting non-vital anterior teeth,
  • Previous hypersensitivity,
  • Under orthodontic treatment,
  • With structural defect in the enamel,
  • Who had used tooth whiteners within the previous 3 years,
  • Smokers,
  • Pregnant or lactating

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
G2 (group 2) - Whitening toothpasteWhitening toothpasteG2 used a whitening toothpaste containing blue pigment in daily oral regimen
G1 (group 1) - Conventional toothpasteConventional toothpasteG1 used a conventional toothpaste in daily oral regimen
G3 (group 3) - 10 % Carbamide peroxide10% carbamide peroxideG3 made an at-home tooth bleaching with 10 % Carbamide peroxide
Primary Outcome Measures
NameTimeMethod
Tooth shade changes from baseline until 4 weeks of the beginning of interventionBaseline, Immediately after first product application, 2 weeks and 4 weeks of the beginning of interventions

The tooth shade evaluation was performed at each time frame using the digital spectrophotometer that provided tooth shade via two different methods: using the 16 shade tabs in the Vita shade guide (B1-C4); and the CIEL\*a\*b\* color system. The difference between the color coordinates was calculated as: ∆E\*= \[(∆L\*)2 + (∆a\*)2 + (∆b\*)2\]1/2, where ΔL\*, Δa\*, and Δb\* are the mathematical differences among CIE L\*, a\*, and b\* at the different time frames and baseline measurements.

Secondary Outcome Measures
NameTimeMethod
Tooth sensitivity and gingival irritation evaluationDaily during three weeks

Each subject should record tooth sensitivity or gingival irritation on a daily basis for 3 weeks (2 weeks of treatment and 1 week after treatment). They should use a standardized grading scale ranked as follows: 0, no sensitivity; 1, mild sensitivity; 2, moderate sensitivity; 3, considerable sensitivity; and 4, severe sensitivity

Subjects' perception about improvement on tooth color2 weeks of the beginning of interventions

After two weeks of treatment, the subjects received a visual analog scale (VAS) ranging from 1 (no improvement in tooth appearance) to 7 (exceptional improvement in tooth appearance), in order to evaluate their satisfaction level with tooth appearance

Acceptability of interventions2 weeks of the beginning of interventions

The acceptability of the three products was assessed using a questionnaire containing six questions about the opinion of subjects regarding the treatment regimen adopted. The questions were scored from positive to negative answers, as follows: 1, agree; 2, neither agree nor disagree; and 3, disagree. The questions related to sufficient instruction, ease of use, comfort level, perceived taste, and overall satisfaction.

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