Pharmacogenomics Validation for Imatinib in Chronic Myeloid Leukemia

Completed

• Conditions: Leukemia, Myelogenous, Chronic, BCR-ABL Positive

• Registration Number: NCT01437202
• Lead Sponsor: Asan Medical Center

Brief Summary

This is a multicenter, retrospective, observational study to validate a pharmacogenetics model for imatinib metabolism and resistance in patients with chronic myeloid leukemia among patients in different independent cohort.

Detailed Description

1. The activity of Imatinib(IM) is mediated by blocking the activity of BCR/ABL tyrosine kinase in CML cells. However, some of the patients failed to achieve optimal response, and a substantial proportion of patients develop resistance to IM.

2. IM is a substrate for the adenosine triphosphate binding cassette (ABC) transporters, ABCB1 and ABCG2, while the active uptake of IM into cells is mediated by the human organic cationic transporter-1 (hOCT1). Also, IM is metabolized through first pass drug metabolism by the cytochrome P450 - CYP3A4 and CYP3A5. In addition, it is delivered in a bound form with a plasma protein referred to α1-acid glycoprotein (AGP).

3. Accordingly, the intracellular or systemic level of imatinib should be influenced by these factors such as ABCB1, ABCG2, hOCT1, CYP3A4, CYP3A5 or AGP genes. Inter-individual variability of 5 candidate genes associated with drug transport/metabolism (i.e. ABCB1, ABCG2, hOCT1, CYP3A4/3A5 and AGP) could affect the expression of corresponding proteins, thus influencing the treatment outcomes of imatinib therapy.

4. In the investigators' previous study, the investigators reported the cumulative incidences of MCyR and CCyR was significantly affected by the predictive model using 2 genotypes and disease stage. These predictive models for CCyR/MMoR or LOR/treatment failure seemed to work well. However, external validation of these predictive models is warranted especially using ethnically different independent cohort.

Study Timeline

• Study Start: 2011-09
• Study First Submitted: 2011-09-19
• Study First Submitted QC: 2011-09-19
• Study First Posted: 2011-09-20
• Primary Completion: 2012-09
• Study Completion: 2012-10
• Status Verified: 2014-07
• Last Update Submitted: 2014-07-08
• Last Update Posted: 2014-07-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Chronic myeloid leukemia of any stage including chronic phase, accelerated or blastic phase.
• Age>18 years
• Complete set of clinical data available for review (demographic data, stage, treatment history, cytogenetic reports, and latest BCR/ABL RQ-PCR results)
• Treated with imatinib mesylate at least 3 months

Exclusion Criteria

• Treated with imatinib mesylate less than 3 months
• Not agree with the intention of this study

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Median time to CCyR (complete cytogenetic response)		Difference of median time to CCyR between cohorts according to the risk stratification by gene analysis

Secondary Outcome Measures

Name	Time	Method
Variance of Genotypes from CML patients with Korean ethnicity
Median time to MCyR (Major cytogenetic responses)		Difference of median time to MCyR between cohorts according to the risk stratification by gene analysis

Trial Locations

Locations (3)

Princess Margaret Hospital, University of Toronto; 🇨🇦 Toronto, Canada

Asan Medical Center, University of Ulsan College of Medicine; 🇰🇷 Seoul, Korea, Republic of

Samsung Medical Center, Sungkyunkwan University School of Medicine; 🇰🇷 Seoul, Korea, Republic of