Microbiological Characterization and Antibiotic Resistance Profiling of Providencia Spp,: An Emerging Pathogen Isolated From Urinary Tract Infections.
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 241
- 试验地点
- 1
- 主要终点
- Determination of prevalence of Providencia species refers specifically to urinary tract infection patients with gram-negative bacterial isolates.
研究概览
简要总结
Aims of the Research:
- Isolation and identification of Providencia spp. from UTIs.
- Genotypic confirmation of Providencia isolates.
- Determination of antimicrobial sensitivity patterns of isolated Providencia spp.
- Characterization of some virulence factors of Providencia spp.
- Molecular characterization of multidrug-resistance gene antibiotics.
- Determination of risk factors for Providencia UTIs.
详细描述
Urinary tract infections (UTIs) are among the most common bacterial infections worldwide, with Escherichia coli, Klebsiella spp., Proteus spp., and Pseudomonas spp. representing the majority of isolated pathogens in both community and hospital settings. Providencia spp., although historically considered uncommon, are increasingly recognized as opportunistic pathogens.
International surveillance studies report that Providencia accounts for ~1-2% of urinary isolates, with higher prevalence (up to 6-12%) in catheter-associated infection.
The Providencia genus, nestled within the Enterobacterales order as part of Proteus and Morganella relatives, consists of Gram-negative bacteria that are adept at producing urease .
The genus includes 14 distinct species, with Providencia rettgeri, Providencia stuartii, Providencia alcalifaciens, and Providencia hangzhouensis standing out due to their pronounced relevance to human infections. Renowned for their association with urinary tract infections, these pathogens also possess the capability to inflict a broader spectrum of severe ailments, such as ocular diseases,peritonitis, neonatal septicemia or bloodstream infections and meningitis.
Providencia stuartii is able to adhere to and invade HeLa-M epithelial cell line.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients with UTI diagnosed through clinical examination and urine analysis. Informed consent will be obtained from each patient before Participation in the study.
排除标准
- •Patients currently on antibiotics.
- •Patients with any anatomical abnormalities in the urinary tract C) Patients having UTI by gram +ve organisms.
结局指标
主要结局
Determination of prevalence of Providencia species refers specifically to urinary tract infection patients with gram-negative bacterial isolates.
时间窗: Through study completion, an average of 1 year
次要结局
未报告次要终点
研究者
Aya Tareq Diab
Teaching assistant at Medical microbiology and Immunology department
Assiut University