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Aging, Nitrate, Endothelial Function and Muscle Oxygenation

Not Applicable
Completed
Conditions
Aging
Abdominal Obesity
Hypertension
Dyslipidemia
Interventions
Other: Beetroot-based nutritional gel
Other: Placebo
Registration Number
NCT02772900
Lead Sponsor
Universidade Federal do Rio de Janeiro
Brief Summary

Aging has been associated with reduced bioavailability of nitric oxide (NO) and endothelial dysfunction. Beetroot consumption, a nitrate-rich food, has been associated with increased NO bioconversion, which may promote beneficial effects on vascular health. The present study evaluated the effects of a beetroot-based nutritional gel (BG) on vascular function, arterial stiffness and blood pressure in the elderly at cardiometabolic risk. Twenty elderly individuals were submitted to BG and nitrate-depleted gel (PLA) interventions. Brachial flow-mediated dilation (FMD), blood flow velocity (BFV), peak wave velocity (PWVβ), augmentation index (AI), stiffness parameter (β), pressure-strain elasticity modulus (Ep), arterial compliance (AC), muscle oxygenation and function were measured 90 min after interventions. Urinary nitrate, nitrite, systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR) were measured at baseline, 90 min and 150 min after interventions.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
25
Inclusion Criteria
  • Elderly participants (≥ 65 years)
  • Taking between 1 and 3 anti-hypertensive medications for high blood pressure;
  • Elevated triglycerides ≥ 150 mg/dL;
  • Reduced HDL-cholesterol < 50 mg/dL for women and < 40 mg/dL for men;
  • Elevated waist circumference by population definition (male: > 102 cm and female: > 88 cm).
Exclusion Criteria

elevated fasting glucose (≥ 100 mg/dL), smoking, beetroot allergy, unwillingness to avoid beetroot products during the entire study, other chronic diseases (diabetes, liver disease, etc.), or acutely ill.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Beetroot gel (dietary nitrate)Beetroot-based nutritional gelBeetroot-based nutritional gel containing approximately 10.0 mmol of nitrate per dose
Placebo gel (nitrate-depleted)PlaceboNutritional gel nitrate-depleted
Primary Outcome Measures
NameTimeMethod
Changes in the arterial stiffness as evaluated by the peak wave velocity.Evaluated 120 min after the nutritional intervention
Changes in endothelial function as evaluated by the flow-mediated dilation.Evaluated 120 min after the nutritional intervention
Changes in endothelial function as evaluated by blood flow velocityEvaluated 120 min after the nutritional intervention
Changes in the arterial stiffness as evaluated by the stiffness parameterEvaluated 120 min after the nutritional intervention
Changes in endothelial function as evaluated by reactive hyperemia.Evaluated 120 min after the nutritional intervention
Changes in the muscle oxygenation as evaluated by the levels of deoxygenated hemoglobinEvaluated 150 min after the nutritional intervention
Changes in the clinical blood pressureEvaluated before, 120 min and 180 min after the nutritional intervention
Changes in the muscle oxygenation as evaluated by the levels of oxygenatedEvaluated 150 min after the nutritional intervention
Changes in the muscle function as evaluated by the maximal voluntary contraction of the forearm muscleEvaluated before and 180 min after the nutritional intervention
Changes in the arterial stiffness as evaluated by augmentation indexEvaluated 120 min after the nutritional intervention
Changes in the arterial stiffness as evaluated by the pressure-strain elasticity modulusEvaluated 120 min after the nutritional intervention
Changes in the arterial stiffness as evaluated by the arterial compliance.Evaluated 120 min after the nutritional intervention
Secondary Outcome Measures
NameTimeMethod
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