Identification of Novel Molecular Pathophysiological Mechanisms of Secondary Brain Injury in Post-cardiac Arrest Syndrome Patients Using Cerebrospinal Fluid Multi-omics Analysis
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Neurological outcome
研究概览
简要总结
The goal of this study is to uncover the molecular mechanisms responsible for secondary brain injury in patients with post-cardiac arrest syndrome by analyzing cerebrospinal fluid (CSF) using multi-omics techniques.
The main question this study aims to answer is:
Which genome-, transcriptome-, proteome-, and metabolome-level changes in CSF are associated with secondary brain injury after cardiac arrest?
To address this question, CSF samples collected from post-cardiac arrest patients will undergo multi-omics analyses. Identified molecular pathways will be used to screen existing drug databases and generate new therapeutic candidates through computational modeling and compound synthesis. These findings will provide the scientific foundation needed to design and implement future preclinical experiments using cardiac arrest animal models.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients receiving post-resuscitation care after out-of-hospital cardiac arrest for secondary brain injury treatment.
- •Patients without contraindications for lumbar puncture catheter insertion for cerebrospinal fluid (CSF) collection. This includes the absence of:
- •Uncontrolled diabetes.Coagulation disorders.
- •Thrombocytopenia (platelet count $< 100,000).
- •A history of cirrhosis diagnosis.
- •Current receipt of low molecular weight heparin.
- •Current use of platelet inhibitors.
- •A history of posterior spinal fusion that may interfere with catheter insertion.
- •Local skin infection or rash at the puncture site.
- •Signs of systemic infection or sepsis.
- •A history of lumbar puncture within the past 6 hours.
排除标准
- •Cerebral Edema: Patients with evidence of cerebral edema on a brain computed tomography (CT) scan performed immediately after spontaneous circulation recovery.
- •Patients who underwent extracorporeal membrane oxygenation (ECMO).
- •Patients who could not maintain integrated therapy for more than 24 hours after cardiac arrest.
- •Patients with a history of acute or chronic brain disease.
研究组 & 干预措施
Favorable neurological outcome
The favorable neurological outcome group is classified as patients with a CPC score of 1 or 2 upon long-term follow-up (at discharge, 3 months after post-resuscitation)
Unfavorable neurological outcome
The favorable neurological outcome group is classified as patients with a CPC score of 3 to 5 upon long-term follow-up (at discharge, 3 months after post-resuscitation)
结局指标
主要结局
Neurological outcome
时间窗: at discharge (assessed up to 3 days), 3 months after return of spontaneous circulation (ROSC)
Neurological outcome assessed using the Cerebral Performance Category (CPC) scale (range 1-5; lower scores indicate better neurological function).
次要结局
未报告次要终点
研究者
Changshin Kang
Assistant professor
Chungnam National University Hospital