Study of individual N-acetyltransferase 1 activity in persons with contact allergy for para-phenylene diamine (PPD).

Completed

• Conditions: allergic contact dermatitis; contact allergie; 10014982

• Registration Number: NL-OMON34651
• Lead Sponsor: niversitair Medisch Centrum Groningen

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 6 May 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 60

Inclusion Criteria

patients: positive patch test on para-phenylene diamine (PPD)
controls: negative patch test on para-phenylene diamine (PPD) and exposure to hairdye in the past

Exclusion Criteria

legally incompetent

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>- N-acetyltransferase 1 (NAT-1) activity within subjects allergic to PPD,<br /><br>compared to subjects without a PPD allergy. The NAT-1 activity will be measured<br /><br>in whole blood, in the whole PBMC population and in the CD3+, CD4+ and CD8+<br /><br>T-cells seperately.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>- Quantification of cytokines in plasma.<br /><br>- Genotyping for the NAT-1 gene and determining the odds ratio of proportional<br /><br>frequencies of NAT-1 variants.<br /><br>- Determining the NAT-1 protein leven in leucocytes/T-cells.<br /><br>- Determining the promotor region methylation status.</p><br>