Study of High Density Lipoprotein Function in Type 1 Diabetic Patients With Nephropathy
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Type 1 Diabetes
- Sponsor
- Centre Hospitalier Universitaire Dijon
- Enrollment
- 200
- Locations
- 1
- Primary Endpoint
- Ability to induce cholesterol efflux
- Status
- Recruiting
- Last Updated
- last year
Overview
Brief Summary
A number of arguments suggest that the deterioration in high density lipoproteins (HDL) functioning may worsen with the development of nephropathy during type 1 diabetes (T1D).
The objective of this study will be to investigate to what extent nephropathy in T1D patients in the microalbuminuria and macroalbuminuria stages, compared to T1D patients without nephropathy, is associated with an alteration in HDL functionality and changes in HDL size and composition (lipids with detailed study of phosphates and sphingolipids, main lipoproteins, inflammatory markers).
Investigators
Eligibility Criteria
Inclusion Criteria
- •INCLUSION CRITERIA GROUP C (CONTROLS) :
- •age \> 18 years
- •a person who has given oral consent
- •Non-diabetics
- •Fasting plasma glucose \< 6.10 mmol/L (1.1 g/L)
- •Triglyceridemia \< 1.7 mmol/L (1.5 g/l)
- •HDL-C concentration \> 1.30 mmol/L (for women and 1.03 mmol/L for men)
- •Glomerular filtration flow rate \> 90 mL/min/1.73m2 (These thresholds correspond to the standard thresholds for these parameters)
- •INCLUSION CRITERIA GROUP T1D-N (WITH NORMAL ALBUMINURIA) :
- •age \> 18 years
Exclusion Criteria
- •Protected adult
- •Patient not affiliated to a social security scheme
- •Pregnant or breastfeeding woman
- •Drugs interfering with lipid metabolism outside insulin for DT1 : hypolipidemic (only for patients included in the DT1-micro group), corticosteroid, estroprogestogens, retinoic acid, antiproteases.
- •BMI \> 30 kg/m2
Outcomes
Primary Outcomes
Ability to induce cholesterol efflux
Time Frame: inclusion
amount of cholesterol captured from cells expressed as %.
Nitric oxide synthesis
Time Frame: inclusion
variation in the presence of HDL compared to the basal state, i.e. in the absence of HDL
Anti-inflammatory effect
Time Frame: inclusion
variation in the expression of adhesion molecules (VCAM-1 \[vascular cell adhesion molecule 1\], ICAM-1 \[InterCellular Adhesion Molecule 1\] and E selectin) under the influence of TNF-alpha \[Tumor Necrosis Factor alpha\] in the presence of HDL compared to the absence of HDL.