Genetic Study of Newly Diagnosed Central Nervous System Tumors in Young Children
- Conditions
- Central Nervous System Embryonal Neoplasm
- Registration Number
- NCT00010114
- Lead Sponsor
- Pediatric Brain Tumor Consortium
- Brief Summary
RATIONALE: Genetic studies may help in understanding the genetic processes involved in the development of some types of cancer.
PURPOSE: Genetic study to learn more about genes involved in the development of central nervous system tumors in young children.
- Detailed Description
OBJECTIVES:
* Identify known genes that have significantly different levels of expression, using microarray gene chip analysis, in infants with newly diagnosed metastatic vs non-metastatic embryonal central nervous system tumors.
* Determine the protein expression of genes identified by microarray analysis that are involved in cellular functions that regulate angiogenesis, invasion, or metastasis in this patient population.
* Determine the quantity of gene expression of the confirmed translationally expressed genes using semi-quantitative polymerase chain reaction.
OUTLINE: This is a multicenter study.
Tumor samples are analyzed using microarray gene chip analysis. Differentially expressed genes are evaluated for protein expression by standard immunohistochemistry and/or Western blot analysis, and gene expression is further quantified by semi-quantitative polymerase chain reaction.
PROJECTED ACCRUAL: Approximately 80-100 patients (20-25 with metastatic disease and 60-75 with non-metastatic disease) will be accrued for this study within 4-5 years.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 83
Not provided
Not provided
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Genes that are expressed in metastatic vs. non-metastatic tumors Prior to therapy
- Secondary Outcome Measures
Name Time Method Protein expression of genes found to be expressed Prior to therapy
Trial Locations
- Locations (9)
Children's National Medical Center
🇺🇸Washington, District of Columbia, United States
Children's Hospital of Philadelphia
🇺🇸Philadelphia, Pennsylvania, United States
UCSF Cancer Center and Cancer Research Institute
🇺🇸San Francisco, California, United States
Dana-Farber Cancer Institute
🇺🇸Boston, Massachusetts, United States
Duke Comprehensive Cancer Center
🇺🇸Durham, North Carolina, United States
Saint Jude Children's Research Hospital
🇺🇸Memphis, Tennessee, United States
Baylor College of Medicine
🇺🇸Houston, Texas, United States
Children's Hospital of Pittsburgh
🇺🇸Pittsburgh, Pennsylvania, United States
Children's Hospital and Regional Medical Center - Seattle
🇺🇸Seattle, Washington, United States