Evaluation of Galectin-3 Levels in GCF And Saliva of Patients With Different Periodontal Diseases

Completed

• Conditions: Periodontal Diseases
• Interventions: Diagnostic Test: GCF obtaining; Diagnostic Test: saliva obtaining

• Registration Number: NCT05281796
• Lead Sponsor: Cairo University

Brief Summary

The aim of this study is to assess the levels of galectin-3 in the GCF and saliva in patient with gingivitis and patient with stage III periodontitis.

Detailed Description

The aim of this study is to assess the levels of galectin-3 in the GCF and saliva in patient with gingivitis and patient with stage III periodontitis.

This study will be conducted in the Oral Medicine and Periodontology department, Faculty of Dentistry- Cairo University, Egypt.

Patients will be selected from the outpatient clinic of the department of Oral Medicine and Periodontology, Faculty of Dentistry-Cairo University.

Study Timeline

• Study Start: 2022-01-22
• Study First Submitted: 2022-03-07
• Study First Submitted QC: 2022-03-15
• Study First Posted: 2022-03-16
• Primary Completion: 2023-06-15
• Study Completion: 2023-08-15
• Status Verified: 2023-11
• Last Update Submitted: 2023-11-22
• Last Update Posted: 2023-11-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 66

Inclusion Criteria

1. For healthy group: individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
2. For the gingivitis group: individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss.
3. For the periodontitis group that has not received periodontal treatment in the last 6 months
4. For the periodontitis group: individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss.
5. Presence of a minimum of 15 natural teeth.

Exclusion Criteria

1. Individuals with any known systemic disease.
2. Pregnant and lactating women.
3. Individuals that received periodontal treatment within the last 6 months.
4. Individuals with a history of systemic antibiotics and anti-inflammatory drugs within the last 3 months.
5. Former or current smokers.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
healthy patient	GCF obtaining	Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained
healthy patient	saliva obtaining	Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained
patients with Stage III periodontitis	GCF obtaining	Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained
gingivitis patient	GCF obtaining	Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained
gingivitis patient	saliva obtaining	Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained
patients with Stage III periodontitis	saliva obtaining	Full-mouth clinical periodontal measurements recorded, saliva and GCF obtained

Primary Outcome Measures

Name	Time	Method
galectin-3 in GCF	24 hours after taking the clinical measurements at the first visit	The total amount of Galectin-3 in GCF
galectin-3 in saliva.	24 hours after taking the clinical measurements at the first visit	The total amount of Galectin-3 in saliva

Secondary Outcome Measures

Name	Time	Method
Plaque Index	24 hours before taking GCF and salivary samples	Scores: 0: No plaque; 1. A film of plaque adhering to the free gingival margin and adjacent area of the tooth. The plaque may be seen insitu only after application of a disclosing solution or by using the probe on the tooth surface.; 2. Moderate accumulation of soft deposit s within the gingival pocket, or the tooth and gingival margin, which can be seen with the naked eye.; 3. Abundance of soft matter within the gingival pocket and/or on the tooth and gingival margin.
Pocket depth	24 hours before taking GCF and salivary samples	in mm
Clinical attachment level	24 hours before taking GCF and salivary samples	in mm
Bleeding on Probing	24 hours before taking GCF and salivary samples	By gently probing the orifice of the gingival crevice, the periodontal probe will be inserted 1 to2 mm into the gingival sulcus starting at one interproximal area and moving to the other. If bleeding occurs within 10 seconds a positive finding is recorded.

Trial Locations

Locations (1)

Cairo university Faculty of dentistry; 🇪🇬 Cairo, Egypt