Evaluation of GCF And Salivary Ferritin, ALP And MMP-8 Levels of Individuals With Different Periodontal Diseases

Not Applicable

Completed

• Conditions: Periodontal Diseases
• Interventions: Diagnostic Test: GCF and saliva obtaining

• Registration Number: NCT06561802
• Lead Sponsor: Izmir Katip Celebi University

Brief Summary

The aim of this clinical study was to determine the levels of ferritin, alkaline phosphatase and matrix metalloproteinase-8 in the gingival crevicular fluid (GCF) and saliva samples were collected from 60 systemically healthy, non-smoker periodontitis (P group, n = 20), gingivitis (G group, n = 20) and periodontally healthy individuals (S group, n = 20) and all clinical parameters were recorded for all groups at baseline. GCF and serum samplings and clinical records were also repeated at 1st and 3rd months visitsfor only P group. Ferritin, MMP8, and ALP levels in samples were determined by enzyme-linked immunosorbent assay (ELISA).

Detailed Description

Not available

Study Timeline

• Study Start: 2019-04-01
• Primary Completion: 2019-09-17
• Study Completion: 2019-09-30
• Status Verified: 2024-08
• Study First Submitted: 2024-08-16
• Study First Submitted QC: 2024-08-16
• Study First Posted: 2024-08-20
• Last Update Submitted: 2024-08-20
• Last Update Posted: 2024-08-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

• Systemically healthy

• At least twenty permanent teeth in the mouth

• Non-smoker

• No medication for continuous use

• Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.

• Not in pregnancy or lactation period.

• For the periodontitis group that has not received periodontal treatment in the last 6 months

  • For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
  • For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
  • For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.

Exclusion Criteria

• Any oral or systemic disease
• Regularly using a systemic medication
• During pregnancy or lactation
• Received periodontal treatment within the last 6 months.
• Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
• Smokers are not included in the study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
periodontitis	GCF and saliva obtaining	Full-mouth clinical periodontal measurements recorded and GCF and saliva obtained.
periodontally healthy	GCF and saliva obtaining	Full-mouth clinical periodontal measurements recorded and GCF and saliva obtained.
gingivitis	GCF and saliva obtaining	Full-mouth clinical periodontal measurements recorded and GCF and saliva obtained.

Primary Outcome Measures

Name	Time	Method
The total amount of ALP in GCF and saliva	24 hours after taking the clinical measurements at the first visit
The total amount of Ferritin in GCF and saliva	24 hours after taking the clinical measurements at the first visit

Secondary Outcome Measures

Name	Time	Method
The total amount of MMP-8 in GCF and saliva	24 hours after taking the clinical measurements at the first visit

Trial Locations

Locations (1)

Izmir Katip Çelebi University Department of Periodontology; 🇹🇷 İzmir, Çiğli, Turkey