Evaluation of Salivary Galectin-8, Galectin-9 and RANKL Levels of Individuals With Different Periodontal Diseases
- Conditions
- Periodontal Diseases
- Interventions
- Diagnostic Test: saliva obtaining
- Registration Number
- NCT06404476
- Lead Sponsor
- Izmir Katip Celebi University
- Brief Summary
The aim of this study is; detection of Galectin-8, Galectin-9 and RANKL levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine Galectin-8, Galectin-9 and RANKL levels in the biological samples.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 60
- Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.)
- At least twenty permanent teeth in the mouth
- Non-smoker
- No medication for continuous use
- Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- Not in pregnancy or lactation period.
- For the periodontitis group that has not received periodontal treatment in the last 6 months
- For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
- For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
- For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
- Any oral or systemic disease
- Regularly using a systemic medication
- During pregnancy or lactation
- Received periodontal treatment within the last 6 months.
- Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
- Smokers are not included in the study
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Gingivitis saliva obtaining Full-mouth clinical periodontal measurements recorded and saliva obtained. Healthy Periodontium saliva obtaining Full-mouth clinical periodontal measurements recorded and saliva obtained. Periodontitis saliva obtaining Full-mouth clinical periodontal measurements recorded and saliva obtained.
- Primary Outcome Measures
Name Time Method The total amount of Galectin-8 in saliva 24 hours after taking the clinical measurements at the first visit The total amount of Galectin-8 in saliva
The total amount of Galectin-9 in saliva 24 hours after taking the clinical measurements at the first visit The total amount of Galectin-9 in saliva
The total amount of RANKL in saliva 24 hours after taking the clinical measurements at the first visit The total amount of Receptor activator of nuclear factor kappaB ligand (RANKL) in saliva
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Izmir Katip Çelebi University
🇹🇷İzmir, Çiğli, Turkey