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Bioequivalence of Rebamipide in Korean

Not Applicable
Completed
Conditions
Therapeutic Equivalency
Pharmacogenetics
Interventions
Drug: Rebamide
Registration Number
NCT00997789
Lead Sponsor
Chonnam National University Hospital
Brief Summary

The aims of this study were to evaluate the pharmacokinetic properties and bioequivalence of two rebamipide preparations in healthy Korean male volunteers for generic substitution and to evaluate the association between the genetic polymorphisms in ABCB1 gene (exon 21 and 26) and rebamipide disposition.

Detailed Description

A randomized, single-dose, 2-period crossover design with a 7-day washout period was conducted in 30 healthy Korean male volunteers. Subjects were randomly assigned to receive a single 100-mg dose of the test or reference preparation of rebamipide, administered with 240 mL of water after a 12-hour overnight fast. All subjects were selected after passing a clinical screening procedure that included a physical examination and laboratory tests. Serum concentrations of rebamipide up to 12 hours after administration were determined using a validated HPLC method with fluorescence detection. Adverse events (AEs) were continuously monitored by clinical staff via observation, personal interview, and vital signs (temperature, blood pressure, heart rate) during the study period. All adverse events were recorded on the clinical record form per subject up to 1 week after the study. Pharmacokinetic parameters were determined using a noncompartmental method. The preparations were considered bioequivalent if the log-transformed ratios of AUC0-t, AUC0-∞, and Cmax were within the predetermined bioequivalence range (ie, 80-125%), as set by the US Food and Drug Administration (FDA) and Korean legislation. In vitro dissolution profiles of both preparations were examined and the influence of genetic polymorphisms in ABCB1 gene (P-glycoprotein) on the pharmacokinetics of rebamipide was also investigated.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
30
Inclusion Criteria
  • Subjects were selected after passing a clinical screening procedure that included a physical examination and laboratory tests (blood analysis; hemoglobin, hematocrit, RBC, WBC, platelet, differential counting of WBC, total proteins, albumin, ALT, AST, alkaline phosphatase, total bilirubin, cholesterol, creatinine, blood urea nitrogen, and fasting glucose and urine analysis; specific gravity, color, pH, sugar, albumin, bilirubin, RBC, WBC and cast).
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Exclusion Criteria
  • Subjects possibly sensitive to this type of preparation,
  • Subjects who had a history of any hepatic illness,
  • Subjects who had a history of any renal illness,
  • Subjects who had a history of any respiratory illness,
  • Subjects who had a history of any endocrine illness,
  • Subjects who had a history of any cardiovascular system illness,
  • Subjects who had taken alcohol within 4 weeks prior to the study,
  • Subjects who had taken other preparations within 4 weeks prior to the study.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Rebamipide, Serum concentration, TabletRebamideThe test preparation, Rebamide® (containing 100 mg of rebamipide; lot No. KP005; expiration date, April 2010; Kyungdong Pharmaceutical Company, Seoul, Korea) and the reference preparation, Mucosta® (containing 100 mg of rebamipide; lot No. MC704067; expiration date, May 2010; Korea Otsuka Pharmaceuticals Co., Ltd., Seoul, Korea)
Primary Outcome Measures
NameTimeMethod
Serum concentration of rebamipidePre-dose (to serve as a control) and at 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8, 10, and 12 hours after oral administration of rebamipide tablet (100 mg)
Secondary Outcome Measures
NameTimeMethod
Genetic polymorphisms in ABCB1 gene (exon 21 and 26)A 3-mL blood sample was taken from each subject who participated in our bioequivalence studies before administration of rebamipide.

Trial Locations

Locations (1)

Institute of Bioeqivalence and Bridging Study, College of Pharmacy, CNU

🇰🇷

Gwangju, Korea, Republic of

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