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Improving Effects of Fish Oil Combined With Pine Bark Extract on Cognitive Decline

Not Applicable
Conditions
Mild Cognitive Impairment
Interventions
Dietary Supplement: FOPE (fish oil + pine extract)
Other: FO (fish oil +placebo)
Registration Number
NCT05573269
Lead Sponsor
Taipei Medical University
Brief Summary

The purpose of this study is to evaluate the improving effects of ω-3 fatty acid from fish oil combined with polyphenolic extract from pine bark (PE) on cognitive functions and biological parameters in healthy people aged 55 to 75.

Detailed Description

Sixty participants who meet the inclusion criteria will be randomly divided into two groups. Participants in FO group will be provided one capsule of fish oil (350mg EPA + 250mg DHA) per day for 6 months. Moreover, participants in FOPE group will be provided one capsule of fish oil (350mg EPA + 250mg DHA) combined with PE (100mg Oligopin) per day for 6 months. A cognitive function evaluation, blood biological analysis and antioxidative status will be carried out at the baseline, 3 th month and the end of the supplementation period. On the other hand, blood fatty acid composition will be measured at the baseline and the end of the supplementation period. All participants will come to laboratory and receive the supplements every month. At the same time, they will accept the measurement of body weight and blood pressure, also be interviewed with 24 hours dietary recall questionnaire.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
60
Inclusion Criteria
  • I. 55~75 years old
  • II. No severe diseases, such as hyperlipidemia, diabetes, heart diseases, cancer etc.
  • III. Mini-mental state examination (MMSE) score is more than 26
  • IV. Clinical dementia rating scale (CDRS) score is less than 0.5
Exclusion Criteria
  • liver disease, kidney disease, hypertension, hyperlipidemia, anemia, cancer

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
FOPE groupFOPE (fish oil + pine extract)Dietary supplement, Polyphenolic extract from pine bark (Oligopin® 100mg) and fish oil capsule contained EPA 350mg + DHA 250mg. This group will receive a nutritional supplement for a period of 6 months. The participants will have a capsule of polyphenolic extract and a capsule of fish oil a day.
FO groupFO (fish oil +placebo)This group will have placebo capsule and fish oil capsule. Placebo capsule contained maltodextrin and magnesium stearate, while fish oil contained EPA 350mg + DHA 250mg. The participants will receive the supplements for a period of 6 months and have a capsule of placebo and a capsule of fish oil a day.
Primary Outcome Measures
NameTimeMethod
Mini-Mental State Examination (MMSE)Change from Baseline and the 12th and 24th week, the experiment will least for 6 months

The most commonly used cognitive function assessment tool in clinical.

Clinical Dementia Rating Scale (CDRS)Change from Baseline and the 12th and 24th week, the experiment will least for 6 months

CDR is a rating scale for staging patients diagnosed with dementia. no dementia (CDR = 0), questionable dementia (CDR = 0.5), MCI (CDR = 1), moderate cognitive impairment (CDR = 2), and severe cognitive impairment (CDR = 3)

Cognitive Ability Screening Instrument (CASI)Change from Baseline and the 12th and 24th week, the experiment will least for 6 months

CASI is a cognitive test screening for dementia. For monitoring the disease progression and providing profiles of cognitive impairment by examining abilities on attention, concentration, orientation, short-term memory, long-term memory, language abilities, visual construction, list-generating fluency and abstraction.

Secondary Outcome Measures
NameTimeMethod
Antioxidative status-SODChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Superoxide dismutase

Kidney function -BUNChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum BUN is in milligram per deciliter

Liver function -ALTChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum ALT is in units per liter.

An outcome related with hematology- plateletChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Platelet in 1000/uL

Antioxidative status-Oxidized LDLChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Oxidized LDL

Kidney function -CreatineChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum creatine is in milligram per deciliter

Nutritional statusChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum albumin is in gram per deciliter

Lipid composition on red blood cell membraneChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Lipid composition on the cell membrane of red blood cell. It will be analyzed by GC/MS system and based on the protocol of Wang et al. The composition of C16:0, C18:0, C18:1, C18:2, C18:3, C20:4, C20:5, C22:5, C22:6, saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, total n-3 fatty acids, total n-6 fatty acids and the ratio of n-3 and n-6 will be measured.

C16:0, C18:0, C18:1, C18:2, C18:3, C20:4, C20:5, C22:5, C22:6, saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, total n-3 fatty acids, total n-6 fatty acids are in percentage.

Kidney function -uric acidChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum uric acid is in milligram per deciliter

Lipid profileChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum HDL-Cho, LDL-Cho, triglyceride and total cholesterol are in milligram per deciliter

Antioxidative status -TBARSChange from Baseline at the 12th and 24th week

Thiobarbituric acid-reactive substance

Liver function -ASTChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum AST is in units per liter.

An outcome related with hematology- white blood cell related measurementsChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

Serum WBC in 1000/uL; neutrophils, lymphocytes, monocytes, eosinophils and basophils are in percentage.

An outcome related with hematology- red blood cell related measurementsChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

RBC in 1000000/uL

Antioxidative status -GSH/GSSGChange from Baseline and the 12th and 24th week, the experiment will least for 6 months

glutathione/oxidized glutathione ratio

Trial Locations

Locations (2)

Taipei Medical University - Shuang Ho Hospital

🇨🇳

New Taipei City, Taiwan

Taiwan Adventist Hospital

🇨🇳

Taipei, Taiwan

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