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Bortezomib Plus Tacrolimus and Methotrexate to Prevent Graft Versus Host Disease (GVHD) After Mismatched Allogeneic Non-Myeloablative Blood Stem Cell Transplantation

Phase 1
Completed
Conditions
Hematologic Malignancies
Interventions
Procedure: blood stem cell transplantation
Registration Number
NCT00369226
Lead Sponsor
Dana-Farber Cancer Institute
Brief Summary

The purpose of this study is to determine if Velcade (also known as bortezomib) can help prevent graft versus host disease (GVHD) developing after transplantation. This is done by using a combination of three immune suppressive medications: Velcade, tacrolimus and methotrexate. Stem cell transplantation is one of the options for patients with cancer of the blood or blood forming organs. Recently, allogeneic stem cell transplants have been performed using lower doses of chemotherapy and radiotherapy: non-myeloablative or "mini" transplants. GVHD is a significant problem that may occur even after "mini" transplantations. Information from other research studies, suggests that Velcade may help to reduce the risk of developing GVHD when given early after transplantation.

Detailed Description

* In this study we are looking for the highest dose of Velcade that can be given to people safely when given with tacrolimus and methotrexate. Not everyone who participates in the study will receive the same amount of the study drug. The dose the participant will receive depends upon the number of subjects enrolled on the study and how well they have tolerated their doses of the drug.

* Before Transplant: In addition to the chemotherapy drugs, fludarabine and busulfex, for the participants non-myeloablative transplant, they will also start taking tacrolimus orally three days before their transplant.

* After Transplant Medication: Methotrexate; Intravenously on days 1, 3, 6 \& 11 after transplant for a total of 4 doses. Tacrolimus; Continue taking orally once daily. Velcade: Intravenously on days 1, 4 \& 7 after transplant, a total of 3 doses. Filgrastim: Subcutaneous injection daily starting the day after transplant and continuing until the participant blood counts have recovered.

* After Transplant Physical Exams \& Tests: Participants will have physical exams and blood tests every week for 1 month. After 1 month, a none marrow biopsy will be performed to look for evidence of the donor's cells in the participants bone marrow.

* Following the 1 month period of time, participants will be seen every few weeks. Another bone marrow biopsy, as well as blood tests, will be taken 3-4 months after the transplant to review the disease status. At this point, participants will come into the clinic about every 3 months, or as determined by their physician for about one year.

* While the study ends at 12 months after transplant, we would like to keep track of the participants medical condition for the rest of their life.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
45
Inclusion Criteria
  • Patients with hematologic malignancies including myelodysplastic syndrome (MDS), who are at a high risk of complications after myeloablative transplantation
  • Patients have a donor (both related and unrelated) who are mismatched according to protocol criteria
  • 18 years of age or older
  • Performance status 0-2
  • Life expectancy of > 100 days
  • Female subject is either post-menopausal or sterilized or willing to use an acceptable form of birth control
  • Male subject agrees to use an acceptable form of birth control
Exclusion Criteria
  • Evidence of HIV infection
  • Total bilirubin > 2.0mg/dl that is due to hepatocellular dysfunction
  • Aspartate aminotransferase (AST) > 90
  • Known active hepatitis B or C
  • Serum creatinine > 2.0
  • Greater than or equal to Grade 2 peripheral neuropathy within 21 days of enrollment
  • Prior allogeneic stem cell transplant
  • Patients with myeloproliferative disease (e.g. myelofibrosis, essential thrombocythemia, polycythemia vera, chronic myeloid leukemia)
  • Myocardial infarction within 6 months prior to enrollment or has NYHA Class III or IV hear failure, uncontrolled angina, severe uncontrolled ventricular arrhythmias, or electrocardiographic evidence of acute ischemia or active conduction system abnormalities
  • Hypersensitivity to Velcade, boron or mannitol
  • Pregnant or breast feeding
  • Patient has received other investigational drugs 14 days before enrollment
  • Serious medical or psychiatric illness
  • Another active solid tumor malignancy at the time of study entry

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Bortezomib/Tacrolimus/Methotrexate post HSCTBortezomib (Velcade)-
Bortezomib/Tacrolimus/Methotrexate post HSCTTacrolimus-
Bortezomib/Tacrolimus/Methotrexate post HSCTMethotrexate-
Bortezomib/Tacrolimus/Methotrexate post HSCTblood stem cell transplantation-
Primary Outcome Measures
NameTimeMethod
The Maximally Tolerated Dose (MTD) of Bortezomib (Velcade) That Can be Administered With Tacrolimus and Methotrexate After Mismatched Allogeneic Non-myeloablative Peripheral Blood Stem Cell (PBSC) Transplantationby day 45 post PBSC infusion

The MTD of bortezomib was evaluated at 3 dose levels:

Dose level 1: 1.0 mg/m\^2 Dose level 2: 1.3 mg/m\^2 Dose level 3: 1.5 mg/m\^2 Cohorts of 3-5 pts were enrolled at each dose level. At any dose level, if no DLT in the first 3, 4, or 5 pts, then dose escalation would occur.

If 3 evaluable pts in cohort, and 1 of 3 experiences DLT then 2 additional pts treated at the same dose level. If \>=1 of 2 additional pts experience DLT then previous dose level will be MTD. If no DLT in additional 2 pts then dose escalation will occur. If 4 evaluable pts in cohort, and 1 of the 4 experiences DLT then 1 additional pt treated at same dose level. If this additional pt experiences DLT then the previous dose will be declared to be the MTD. If additional pt does not experience DLT, then dose escalation will take place. If 5 evaluable pts in cohort, and 1 experiences DLT, then dose escalation will take place. If \>=2 of first 3, 4, or 5 pts experience DLT then the previous dose will be declared MTD.

Successful Initial Engraftment by Day 45 Post Peripheral Blood Stem Cell (PBSC) Infusion and Administration of Bortezomib (Velcade), Tacrolimus and Methotrexateby day 45 post PBSC infusion

Percentage of participants who did not experience failure to engraft or relapse or death before assessment.

Incidence of Grade II-IV Acute Graft Versus Host Disease (GVHD) by Day 100.by day 100 after peripheral blood stem cell (PBSC) infusion
Secondary Outcome Measures
NameTimeMethod
Sustained Engraftment Following Transplant.by day 100 post transplant

As measured by median total donor chimerism at day 100.

Incidence of Chronic Graft Versus Host Disease (Chronic GVHD).by 1 year after PBSC infusion

Number of participants with chronic GVHD at 1 year post transplant.

Overall Survival and Progression-free Survival.by 1 year after PBSC infusion

Progression is defined as disease relapse or disease progression since transplant.

Trial Locations

Locations (2)

Dana-Farber Cancer Institute

🇺🇸

Boston, Massachusetts, United States

Brigham and Women's Hospital

🇺🇸

Boston, Massachusetts, United States

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