Examining The Effect of Blood Flow Restricted Aerobic Training on Mitochondrial Adaptations in Young Healthy Adults

Not Applicable

Completed

• Conditions: Mitochondrial Biogenesis
• Interventions: Other: CTL Exercise Training; Other: BFR Exercise Training

• Registration Number: NCT04151095
• Lead Sponsor: Brendon Gurd, PhD

Brief Summary

This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2019-04-02
• Study Start: 2019-05-06
• Study First Submitted QC: 2019-11-01
• Study First Posted: 2019-11-05
• Primary Completion: 2019-12-13
• Study Completion: 2019-12-13
• Status Verified: 2023-12
• Last Update Submitted: 2023-12-19
• Last Update Posted: 2023-12-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 19

Inclusion Criteria

• 3 hours of physical activity per week
• No previous cycling training
• No concurrent involvement in another training program
• Body mass index < 30 kg/m2

Exclusion Criteria

• Presence of cardiovascular disease
• Presence of metabolic disease
• Taking regular oral medication
• Current smoker

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
CTL	CTL Exercise Training	-
BFR	BFR Exercise Training	-

Primary Outcome Measures

Name	Time	Method
Citrate Synthase	3 days prior to start of exercise training and 3 days after completing exercise training	Fold change in citrate synthase maximal activity

Secondary Outcome Measures

Name	Time	Method
Near-infrared spectroscopy (NIRS)	Throughout each of the 7, 30-minute exercise sessions	Deoxygenated hemoglobin and oxygenated hemoglobin
Transcription factor A, mitochondrial (TFAM)	3 days before training to 3 days after training	Fold change in TFAM protein
Maximal oxygen consumption (VO2peak)	3 days before training to 3 days after training	Change in VO2peak (POST-PRE)
Revolutions per minute (RPM)	Every 5 minutes during each of the 7, 30-minute exercise sessions	Average RPM
Blood [lactate]	During all exercise sessions at 10 and 30 minutes	Average blood lactate concentration
Cytochrome c oxidase (COX)	3 days before training to 3 days after training	Fold change in COX
Electromyography (EMG)	Throughout each of the 7, 30-minute exercise sessions	Median frequency and average amplitude of EMG signal
Nuclear respiratory factor, 1 (NRF1)	3 days before training to 3 days after training	Fold change in NRF-1 protein
Rating of perceived exertion (RPE)	Every 5 minutes during each of the 7, 30-minute exercise sessions	Average RPE
Nuclear Factor, Erythroid 2 Like 2 (NFE2L2)	3 days before training to 3 days after training	Fold change in NFE2L2
Heart rate (HR)	Every 5 minutes during each of the 7, 30-minute exercise sessions	Average HR

Trial Locations

Locations (1)

Queen's University; 🇨🇦 Kingston, Ontario, Canada